Objective: To investigate the mechanism of action of Shengmaisan. Method: Potential targets related to 33 active chemical components from Ginseng Radix et Rhizoma

Ophiopogonis Radix and Schisandrae Chinensis Fructus of Shengmaisan were predicted through PharmMapper server. Compound-target network

protein-protein interaction (PPI) network

gene ontology (GO) enrichment analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis were constructed to explore the mechanism of action of Shengmaisan. Result: Compound-target network contained 249 nodes

in which the key targets involved Transthyretin (TTR)

etc. PPI network contained 155 nodes

in which the key targets involved Proto-oncogene tyrosine-protein kinase Src (SRC)

RAC-alpha serine/threonine-protein kinase (Akt1)

etc. In GO enrichment analysis

there were 95 GO terms

including 67 terms related to biological process

22 related to molecular function and 6 related to cellar components. In KEGG pathway enrichment analysis

there were 8 KEGG pathways

involving pathways in cancer

prostate cancer

insulin signaling pathway and so on. Conclusion: The results of the study have preliminarily verified the basic pharmacological effects and related mechanisms of Shengmaisan

and laid a solid foundation for further studies on the mechanism of action of Shengmaisan.