Objective: To prepare paeonol self-microemulsion system and evaluate its transdermal absorption characteristics. Method: The pseudo-ternary phase diagram was prepared by adding the emulsifier titration method

the types and components of emulsifiers and co-emulsifier were screened

and the maximum drug loading of paeonol self-microemulsion was determined.The appearance of the microemulsion was observed by transmission electron microscopy (TEM).The particle size distribution and Zeta potential of the microemulsion were determined by laser scattering particle size analyzer.The transdermal absorption characteristics of paeonol self-microemulsion were investigated by Franz diffusion cell method. Result: Taking peppermint oil as the oil phase

tween-80 as the emulsifier and absolute ethanol as the co-emulsifier

and the ratio of these components was 2: 6: 2.The maximum drug loading of paeonol self-microemulsion was 100 mg · g-1

its appearance was regular spherical

the average particle size was (63.2±0.27) nm and the Zeta potential was (0.14±0.29) mV.The cumulative release amount of paeonol in the self-microemulsion was 589.10 μg · cm-2 per unit area at 12 h. Conclusion: The prepared paeonol self-microemulsion system is clear and transparent with stable properties

its particle size is suitable

which is in accordance with the requirement of self-microemulsion preparation

and the transdermal absorption effect of this preparation is good.