Objective: To investigate the efficacy differences of various combinations of Chuanxiong Rhizoma-Gastrodiae Rhizoma in treatment of blood stasis type migraine. Method: Total 42 SD rats were randomly divided into 7 groups: normal group (Z)

model group (M)

sham-operated group (J)

Chuanxiong Rhizoma-Gastrodiae Rhizoma groups A (1: 4)

B(4: 1)

C(1: 1)

and Sumatriptan group positive control group(Y). In groups A

B

C

intragastric administration of Chuanxiong Rhizoma-Gastrodiae Rhizoma concentrated solution at 20 mL · kg-1 · d-1 was given for continuous 8 weeks; in groups Z and J

water and sumatriptan solution at 20 mL · kg-1 · d-1 was given respectively for 8 weeks by intragastric administration. SD rats received subcutaneous injection of hydrochloride (0.8 mg · kg-1) and trinitrin(10 mg · kg-1) after ice bathing to establish blood stasis type margarine models. Calcitonin gene related peptide (CGRP)

thromboxane B2 (TXB2)

6-keto-prostaglandin F1 alpha (6-Keto-PGF1α) in serum and nitric oxide (NO)

nitric oxide synthase (NOS)

CGRP

endothelin (ET) in brain tissues were detected by enzyme linked immunosorbent assay (ELISA). Result: As compared with the normal group

the levels of NO

NOS

CGRP

6-Keto-PGF1α and the ratio of NO/ET in serum

and the levels of NO

NOS

ET

CGRP

TXB2 and 6-Keto-PGF1α in brain tissues were increased in model group (P<0.05). As compared with the model group

NO/ET

CGRP and 6-Keto-PGF1α in serum were reduced (P<0.05) in Chuanxiong Rhizoma-Gastrodiae Rhizoma 4: 1 group

TXB2 content and TXB2/6-Keto-PGF1α ratio were increased (P<0.05). Chuanxiong Rhizoma-Gastrodiae Rhizoma 4: 1 group could reduce NO

ET

TXB2

6-Keto-PGF1α levels in brain tissues and NOS activity (P<0.05)

and increase the ratio of TXB2/6-Keto-PGF1α (P<0.05) in brain tissues. Conclusion: All the combination groups were effective for blood stasis type migraine

and group(4: 1) had the best efficacy in consideration of multiple indexes.