Objective: To explore the effect of hyperoside (Hyp) on the damaged blood-brain barrier (BBB) induced by amyliod-beta protein (Aβ1-42) in vitro model. Method: In this experiment

Hyp intervention was used for the injury of fibrous Aβ1-42-mediated bEnd.3 cells and primary astrocytes (astrocytes

As) BBB co-culture models. By detecting the permeability of sodium fluorescein in various culture groups

BBB permeability changes were compared between various groups to evaluate the effect of Hyp on permeability of Aβ1-42-mediated BBB; methyl thiazolyl tetrazolium (MTT) was used to detect cells vitality and uncover the effect of Hyp on it; then in order to explore its protection mechanism

the apoptosis related proteins were detected by Western blot. Result: As compared with the model group

BBB permeability was reduced in Hyp low

middle and high dose groups (P<0.05

P<0.01); bEnd.3 cells activity was improved in Hyp middle and high dose groups (P<0.05); Western blot results showed that the ratio of Bax/Bcl-2

cleaved cysteine aspartic acid protease-3(Caspase-3)/Caspase-3 and the expression levels of cytochrome-C (Cyt-C) were declined obviously in the Hyp groups

and the differences were statistically significant (P<0.05). Conclusion: Hyp could inhibit the fibrous Aβ1-42-induced permeability increase of BBB via protecting cerebral microvascular endothelial cells..