Objective: To prepare resveratrol liposomes and evaluate its anti-tumor effect in vitro.Method: The resveratrol liposomes were prepared by film dispersion-ammonium sulfate gradient method and the free resveratrol was filtered by sephadex G-50 column afterwards.Moreover

the particle size and Zeta potential of liposomes were characterized by laser particle size analyzer.C6 glioma cells were cultured for 4 days and their logarithmic growth phase was decided by cell growth curve.These cells were tested in several experiments in vitro as below.The antiproliferative effect of free resveratrol on C6 glioma cells were evaluated by sulforhodamine B(SRB) method

while the antiproliferative effect of resveratrol liposomes on C6 glioma cells were tested by the same way.Furthermore

the apoptosis effect on C6 glioma cells treated by free resveratrol were tested by flow cytometry method.Also

the apoptosis effect on C6 glioma cells treated by resveratrol liposomes were observed by the same way.The uptake effect of resveratrol and resveratrol liposomes on C6 glioma cells were all determined by this method.Result: The particle size of blank liposomes and resveratrol liposomes were separately (144.57±0.93) nm and (139.97±0.64) nm

while their Zeta potentials were respectively (-6.70±0.93) mV and (-7.00±0.74) mV.The logarithmic growth phase of C6 glioma cells was between 36 h to 48 h.The SRB method showed that the inhibitory effect on C6 glioma cells treated by resveratrol liposomes was (91.70±0.60)%

which was significantly higher than(73.30±0.56)% of free resveratrol(P<0.05).The apoptosis effect on C6 glioma cells induced by resveratrol liposomes was (27.18±0.96)%

which was obviously higher than (20.03±0.85)% of free resveratrol(P<0.05).The uptake effect of resveratrol liposomes was (77.61±1.67)%

which was also significantly higher than (67.79±1.19)% of free resveratrol (P<0.05).Conclusion: Compared with free resveratrol

resveratrol liposomes has a more significant antiproliferative effect against C6 glioma cells

and has a stronger apoptosis effect on C6 glioma cells.The number of resveratrol liposomes that uptake is obviously more than that of free resveratrol.It is concluded that the resveratrol liposomes built in this study shows strongest anti-tumor effect on C6 glioma cells in vitro by comparing with other controlled groups.