Objective: To observe the effect of Yiguanjian on growth of liver cancer

Janus protein tyrosine kinase/signal transducers and transcriptional activator proteins (JAK1/STAT1) signaling pathway

as well as the expression of downstream apoptosis related proteins C-myc

B-cell lymphoma-2 (Bcl-2)

and p53.Method: The 50 male KM mice were randomly divided into model group

cyclophosphamide[CTX

50 mg · kg-1 · (2 d)-1] group

and Yiguanjian high

middle

and low dose groups (46

23

11.5 g · kg-1 · d-1). H22 tumor cells were injected under armpit to establish tumor-bearing mice models. Treatment began two weeks before modeling in Yiguanjian high

medium and low dose groups. CTX was administrated after modeling and the mice in model group were administrated with the same volume of normal saline after modeling 2 weeks before and after modeling. The mice in various groups were sacrificed

and their tumor tissues were taken and weighed. Then the JAK1 and STAT1 protein phosphorylation levels were detected by immunohistochemistry and C-myc

Bcl-2 and p53 protein expression levels were detected by protein electrophoresis.Result: As compared with model group

there was a significant inhibition effect on tumors in Yiguanjian high

medium

low dose groups and CTX group (P<0.01); high dose and middle dose Yiguanjian could significantly reduce JAK1 protein phosphorylation level (P<0.05) and significantly increase STAT1 protein phosphorylation level (P<0.05); high dose Yiguanjian could significantly reduce protein expression of C-myc and promote the protein expression of p53 (P<0.05); high and middle dose Yiguanjian could significantly decrease the protein expression of Bcl-2 (P<0.05

P<0.01).Conclusion: Yiguanjian had obvious antitumor effect probably by regulating JAK1/STAT1 phosphorylation

promoting its downstream p53 protein expression

inhibiting its downstream C-myc

Bcl-2 protein expression

and promoting apoptosis of liver cancer cells.