双益方、组方中药及有效成分对骨骼肌及HepG2细胞胰岛素抵抗的影响

田春雨; 薄海美; 喇孝瑾; 朱亮; 贾永森; 曹慧娟; 李继安; 张碧溦; 徐雪梅; 李孟佳

doi:10.13422/j.cnki.syfjx.2017180118

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双益方、组方中药及有效成分对骨骼肌及HepG2细胞胰岛素抵抗的影响

药理 | 更新时间：2024-01-04

- 双益方、组方中药及有效成分对骨骼肌及HepG2细胞胰岛素抵抗的影响
- Effect of Shuangyi Prescription, Component Herbs and Different Active Ingredients on Insulin Resistance in Skeletal Muscle and HepG2 Cells
- 中国实验方剂学杂志 2017年23卷第18期页码：118-123
- 作者机构：
- 作者简介：
- 基金信息：
  
  河北省自然科学基金项目（H2015209025）;河北省高等学校科学技术研究项目（QN2015119）
- DOI：10.13422/j.cnki.syfjx.2017180118
  中图分类号： R285
- 纸质出版日期：2017
- 稿件说明：
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田春雨, 薄海美, 喇孝瑾, 等. 双益方、组方中药及有效成分对骨骼肌及HepG2细胞胰岛素抵抗的影响[J]. 中国实验方剂学杂志, 2017,23(18):118-123.

TIAN Chun-yu, BO Hai-mei, LA Xiao-jin, et al. Effect of Shuangyi Prescription, Component Herbs and Different Active Ingredients on Insulin Resistance in Skeletal Muscle and HepG2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(18): 118-123.
田春雨, 薄海美, 喇孝瑾, 等. 双益方、组方中药及有效成分对骨骼肌及HepG2细胞胰岛素抵抗的影响[J]. 中国实验方剂学杂志, 2017,23(18):118-123. DOI： 10.13422/j.cnki.syfjx.2017180118.

TIAN Chun-yu, BO Hai-mei, LA Xiao-jin, et al. Effect of Shuangyi Prescription, Component Herbs and Different Active Ingredients on Insulin Resistance in Skeletal Muscle and HepG2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(18): 118-123. DOI： 10.13422/j.cnki.syfjx.2017180118.

摘要

目的：研究双益方、组方中药及有效成分对骨骼肌细胞胰岛素抵抗模型葡萄糖消耗量及人肝癌HepG2细胞糖原合成，己糖激酶（hexokinase，HK），丙酮酸激酶（pyruvate kinase，PK）活性的影响，初步探讨其作用机制，为其临床应用奠定基础。方法：采用高胰岛素方法建立骨骼肌细胞的胰岛素抵抗（IR）模型，观察双益方低、中、高剂量组、组方中药（黄芪、山茱萸、黄连、桑白皮、葛根、佩兰），有效成分（黄芪甲苷，毛蕊异黄酮葡萄糖苷，熊果酸，马钱苷，小檗碱，1-脱氧野尻霉素，葛根素）组（30，120，480 μg · L-1）对骨骼肌细胞胰岛素抵抗模型的葡萄糖消耗量的影响，蛋白质免疫印迹法（Western blot）测定骨骼肌细胞蛋白酪氨酸磷酸酶-1B（protein tyrosine phosphatase-1B，PTP-1B），葡萄糖转运蛋白4（glucosetransporters 4，GLUT4）表达；建立HepG2细胞的胰岛素抵抗模型，研究双益方、组方中药及其有效成分对HepG2细胞糖原合成及己糖激酶、丙酮酸激酶活性的影响。结果：双益方，黄芪，山茱萸，黄芪甲苷，1-脱氧野尻霉素，葛根素可降低骨骼肌细胞细胞上清液葡萄糖浓度（P<0.05），以480 μg · L-1双益方，黄芪甲苷组及葛根素组效果较好；与模型组比较，双益方、黄芪及有效成分组方可降低PTP-1B，提高GLUT-4的表达（P<0.05，P<0.01）。与正常组比较，模型组糖原合成量及HK，PK活性显著降低（P<0.01）；与模型组比较，各浓度组单味中药及有效成分均能一定程度的增加糖原合成量及HK，PK活性（P<0.05，P<0.01），其中黄芪甲苷、黄芪提取液增加糖原合成及提高HK活性作用较好；双益方提取液及葛根素增加PK活性作用较好。结论：双益方、组方中药及有效成分对骨骼肌，HepG2细胞胰岛素抵抗具有一定缓解作用，降低PTP-1B表达、提高GLUT4表达、增加糖原合成、提高HK，PK活性可能是其作用机制。

Abstract

Objective: To study the effect of Shuangyi prescription (SYP)

component herbs and active ingredients on insulin resistance model of skeletal muscle cells

and glycogen synthesis

hexokinase (HK) and pyruvate kinase (PK) activity of IR-HepG2 cells

in order to analyze the mechanism

and lay a foundation for its clinical application.Method: The insulin resistance model of skeletal muscle cells was established by high concentration of insulin

in order to observe the effect of low

medium and high-dose (30

120

480 μg · L-1) SYP

component herbs (Astragali Radix

Corni Fructus

Coptidis Rhizome

Mori Cortex

Puerariae Lobatae Radix

Eupatorii Herba)

and different active ingredients (astragaloside

calycosin-7-glucoside

ursolic acid

loganin

berberine

puerarin

1-deoxynojirimycin) on insulin resistance model of skeletal muscle cells. The IR-HepG2 cells model of high concentration of insulin was established in vitro

so as to study the effects of glycogen synthesis

HK and PK activities of IR-HepG2 cells of SYP

component herbs and active ingredients. Western blot was performed to detect protein expressions of tyrosine 4 phosphatase-1B(PTP-1B) and glucose transporters 4(GLUT4).Result: SYP

Astragali Radix

Corni Fructus

astragaloside

1-deoxynojirimycin

and puerarin could reduce glucose concentration in skeletal muscle cell supernatant (P<0.05)

with a better effect in SYP

Astragali Radix and puerarin groups with the dose of 480 mg · L-1. Compared with the model group

SYP

Astragali Radix and active ingredients could decrease the expression of PTP-1B

and increase the expression of GLUT-4.Compared with the model group

different concentrations of active ingredients and component herbs could increase glycogen synthesis and HK

PK activities of HepG2 cells(P<0.05

P<0.01); Astragaloside and Astragali Radix extracts had a better effect in increasing glycogen synthesis and HK activity

and SYP and puerarin had a better effect in increasing PK activity.Conclusion: SYP

component herbs and active ingredients could relieve the insulin resistance of skeletal muscle and HepG2 cells

and its action mechanism may be decrease in the expression of PTP-1B

and increase in the expression of GLUT4

glycogen synthesis and HK

PK activities.

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