Objective: To evaluate the correlation between spleen deficiency and liver stagnation

and brain gut peptides in rats with diarrhea-predominant irritable bowel syndrome (D-IBS).Method: A rat model of D-IBS was established through intragastric administration with Sennae Folium decoction combined with restraint stress. Rats were divided into several groups. The modeling groups were given Sennae Folium decoction combined with restraint stress. The colonic motility

visceral sensitivity and the expression of brain gut peptides were measured at 0 d

7th d

14th d

21st d

28th d after modeling. Maclab/4e 4-channel physiology recorder was used to record colon motion curves

and evaluate colonic motility index. The visceral sensitivity of rats was evaluated by colorectal distention

and water injection rate was recorded when the abdominal withdrawal reflex (AWR) was scored at 3.Enzyme linked immunosorbent assay (ELISA)

immunohistochemistry assay and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the expression of brain gut peptides.Result: In 14th d

21st d

28th d groups

the colonic motility index

the water injection rate when AWR was scored at 3

the expressions of 5-HT

CRH

5-HT and CRH mRNA in colon and hippocampus were significantly different (P<0.05

P<0.01) from before modelling. In 21st d

28th d group

the colonic motility index

the motility index change rate

the water injection rate when AWR was scored at 3

the expressions of 5-HT

CRH

5-HT and CRH mRNA in colon and hippocampus were significantlydifferent (P<0.05

P<0.01) from those in the 7th d group. In 21st d

28th d groups

the water injection rate when abdominal withdrawal reflex (AWR) was scored at 3

the expressions of 5-HT

CRH

5-HT and CRH mRNA in colon and hippocampus were significantly different (P<0.05

P<0.01) from those in the 14th d group. The colonic motility index and the motility index change rate in 28th d group were significantly different from those the 14th d group (P<0.05

P<0.01).Conclusion: The 5-HT and CRH are closely correlated with CRH and D-IBS with spleen deficiency and liver stagnation symptoms

which might be the material basis of spleen deficiency and liver stagnation syndrome in D-IBS.