血腑逐瘀胶囊对缺血心肌细胞SIRT1信号转导通路的干预作用

段锦龙; 姚魁武; 刘张静; 陈孟倩

doi:10.13422/j.cnki.syfjx.2017180141

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血腑逐瘀胶囊对缺血心肌细胞SIRT1信号转导通路的干预作用

药理 | 更新时间：2024-01-04

- 血腑逐瘀胶囊对缺血心肌细胞SIRT1信号转导通路的干预作用
- Effect of Xuefu Zhuyu Capsule on SIRT1 Signal Transduction Pathway of Ischemic Cardiomyocytes
- 中国实验方剂学杂志 2017年23卷第18期页码：141-144
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81473466）
- DOI：10.13422/j.cnki.syfjx.2017180141
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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段锦龙, 姚魁武, 刘张静, 等. 血腑逐瘀胶囊对缺血心肌细胞SIRT1信号转导通路的干预作用[J]. 中国实验方剂学杂志, 2017,23(18):141-144.

DUAN Jin-long, YAO Kui-wu, LIU Zhang-jing, et al. Effect of Xuefu Zhuyu Capsule on SIRT1 Signal Transduction Pathway of Ischemic Cardiomyocytes[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(18): 141-144.
段锦龙, 姚魁武, 刘张静, 等. 血腑逐瘀胶囊对缺血心肌细胞SIRT1信号转导通路的干预作用[J]. 中国实验方剂学杂志, 2017,23(18):141-144. DOI： 10.13422/j.cnki.syfjx.2017180141.

DUAN Jin-long, YAO Kui-wu, LIU Zhang-jing, et al. Effect of Xuefu Zhuyu Capsule on SIRT1 Signal Transduction Pathway of Ischemic Cardiomyocytes[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(18): 141-144. DOI： 10.13422/j.cnki.syfjx.2017180141.

摘要

目的：观察血腑逐瘀胶囊对大鼠缺血心肌细胞Sirt1信号通路的干预作用，探讨该中药复方的作用机制。方法：将70只健康成年雌性Wistar大鼠随机分成正常组，假手术组（以蒸馏水10 mL ·kg-1灌胃），模型组（以蒸馏水10 mL ·kg-1灌胃），血腑逐瘀高、中、低剂量组（0.03，0.02，0.01 g ·kg-1），L-NAME组（按2 mg/只腹腔注射），采用实时荧光定量PCR（Real-time PCR）技术检测各组心肌组织沉默信息调节因子1（Sirt1），p53及核转录因子-κB（NF-κB） mRNA表达情况。结果：与假手术组比较，模型组缺血心肌细胞Sirt1 mRNA表达水平明显降低（P<0.05），缺血心肌细胞p53，NF-κB mRNA表达水平增高（P<0.05）；与模型组比较，血腑逐瘀高、中、低剂量组大鼠缺血心肌细胞Sirt1 mRNA表达水平明显增高（P<0.05），血腑逐瘀高剂量组大鼠缺血心肌细胞p53，NF-κB mRNA表达水平明显降低（P<0.05）。结论：血腑逐瘀胶囊可延长缺血心肌细胞寿命，其机制可能是通过激活Sirt1信号通路，抑制p53，NF-κB基因的表达而发挥作用。

Abstract

Objective: To investigate the effect of Xuefu Zhuyu capsule on Sirt1 signal transduction pathway of ischemic cardiomyocytes in rats

and discuss its mechanism of action.Method: A total of 70 healthy adult female wistar rats were randomly divided into normal group

sham operation group (10 mL · kg-1 distilled water)

model group (10 mL · kg-1 distilled water)

Xuefu Zhuyu high dose group (0.03 g · kg-1)

Xuefu Zhuyu middle dose group (0.02 g · kg-1)

Xuefu Zhuyu low dose group (0.01 g · kg-1)

and L-NAME group (2 mg/rat

intraperitoneal injection). The mRNA expression levels of Sirt1

p53 and NF-κB in myocardium were detected by real-time fluorescence quantitative PCR.Result: As compared with the sham operation group

the expression level of Sirt1 was significantly reduced in the model group (P<0.05); the expression levels of p53 and NF-κB in myocardium were increased in model group(P<0.05). As compared with the model group

the expression level of Sirt1 in ischemic myocardium was increased in Xuefu Zhuyu high

middle and low dose groups (P<0.05); the expression levels of p53 and NF-κB in myocardium were decreased in Xuefu Zhuyu high dose group (P<0.05).Conclusion: Xuefu Zhuyu capsule can inhibit the expression of p53 and NF-κB genes by activating Sirt1 signaling pathway to exert its anti-aging effect and prolong the life span of ischemic cardiomyocytes.

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