丹参多酚酸盐干预非ST段抬高心肌梗死患者microRNA与靶基因

张振鹏; 滕菲; 刘咏梅; 李军; 王阶

doi:10.13422/j.cnki.syfjx.2017190017

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丹参多酚酸盐干预非ST段抬高心肌梗死患者microRNA与靶基因

冠心病病证结合分子生物研究专题 | 更新时间：2024-01-04

- 丹参多酚酸盐干预非ST段抬高心肌梗死患者microRNA与靶基因
- Intervention Effect of Salvianolate on microRNA and Target Genes in Patients with Non-ST-segment Elevation Myocardial Infarction
- 中国实验方剂学杂志 2017年23卷第19期页码：17-22
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81473561）
- DOI：10.13422/j.cnki.syfjx.2017190017
  中图分类号： R259
- 纸质出版日期：2017
- 稿件说明：
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张振鹏, 滕菲, 刘咏梅, 等. 丹参多酚酸盐干预非ST段抬高心肌梗死患者microRNA与靶基因[J]. 中国实验方剂学杂志, 2017,23(19):17-22.

ZHANG Zhen-peng, TENG Fei, LIU Yong-mei, et al. Intervention Effect of Salvianolate on microRNA and Target Genes in Patients with Non-ST-segment Elevation Myocardial Infarction[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(19): 17-22.
张振鹏, 滕菲, 刘咏梅, 等. 丹参多酚酸盐干预非ST段抬高心肌梗死患者microRNA与靶基因[J]. 中国实验方剂学杂志, 2017,23(19):17-22. DOI： 10.13422/j.cnki.syfjx.2017190017.

ZHANG Zhen-peng, TENG Fei, LIU Yong-mei, et al. Intervention Effect of Salvianolate on microRNA and Target Genes in Patients with Non-ST-segment Elevation Myocardial Infarction[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(19): 17-22. DOI： 10.13422/j.cnki.syfjx.2017190017.

摘要

目的：高通量检测非ST段抬高性心肌梗死（NSTEMI）患者药物干预前后的微小RNA（microRNA）表达谱，对比中西药物治疗前后microRNA表达变化，分析活血化瘀药物干预靶标与机制。方法：选取符合西医疾病诊断标准和中医证侯诊断标准的患者，分为2组，包括NSTEMI中药治疗组，NSTEMI西药组，每组纳入35例，抽取外周血，分离外周血单核细胞（PBMC），提取总RNA。根据各组差异表达的microRNA和基因的通路和网络分析结果，确定各组中起关键调控作用的microRNA及其靶基因。结果：在microRNA表达谱中，中药治疗组治疗前后、中药治疗组与西药组两组治疗后比较均存在差异；中药治疗组治疗后hsa-miR-92a，hsa-miR-363，hsa-miR-499，hsa-miR-30b，hsa-miR-454-3p，hsa-miR-933表达下调，hsa-miR-144，hsa-miR-451，hsa-miR-494，hsa-miR-320表达上调。表明这10个microRNA是丹参多酚酸盐干预NSTEMI的靶标。实时荧光定量PCR（Real-time PCR）验证，与西药组比较，中药治疗组治疗后hsa-miR-92a，hsa-miR-30b，hsa-miR-499，hsa-miR-454表达下调；中药治疗组治疗后hsa-miR-494的表达上调（P<0.05）。结论： NSTEMI患者治疗前后，中药治疗组与西药组microRNA的表达谱均存在差异，筛选的miRNA可能是活血化瘀药物干预的靶标。

Abstract

Objective: To conduct high throughput detection of microRNA expression in patients with non-ST segment elevation myocardial infarction (NSTEMI) before and after drug intervention

compare the expression changes of microRNA before and after treatment with Chinese and western medicines

and analyze the intervention targets and mechanism of Chinese medicines for promoting blood flow and eliminating blood stasis. Method: Patients who met the diagnostic criteria of Western medicine diseases and the diagnostic criteria of traditional Chinese medicine (TCM) syndromes were divided into NSTEMI Chinese medicine treatment group and NSTEMI western medicine control group

35 cases in each group. Their peripheral blood was extracted

peripheral blood mononuclear cells (PBMC) were isolated and total RNA was extracted. The active microRNA and its target genes in each group were identified according to differentially expressed microRNAs

gene pathways and network analysis results. Result: In the microRNA expression profile

there were differences between Chinese medicine treatment group before and after treatment as well as between TCM treatment group and western medicine control group after treatment

hsa-miR-92a

hsa-miR-363

hsa-miR-499

hsa-miR-30b

hsa-miR-454-3p

and hsa-miR-933 expression levels were down-regulated

while hsa-miR-144

hsa-miR-451

hsa-miR-494

and hsa-miR-320 expression levels were up-regulated in TCM treatment group after treatment. These 10 microRNAs were considered as the intervention targets of salvianolate for NSTEMI. Real-time PCR

results verified that as compared with the western medicine control group

hsa-miR-92a

hsa-miR-30b

hsa-miR-499 and hsa-miR-454 expression levels were down-regulated while the expression of hsa-miR-494 was up-regulated after treatment in Chinese medicine treatment group (P<0.05). Conclusion: There are differences in the expression profiles of microRNAs between the Chinese medicine treatment group and the western medicine control group before and after the treatment of NSTEMI

and the screened miRNAs may be the intervention targets for Chinese medicines of promoting blood flow and eliminating blood stasis.

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