Objective: To identify the differentially expressed lncRNA

miRNA and mRNA levels related to coronary heart disease (CHD) blood stasis syndrome

construct the interaction network

and explore the material foundation and mechanism of blood stasis syndrome from the perspective of transcriptomics. Method: The expression levels of lncRNA

miRNA and mRNA in CHD blood stasis syndrome group

non-blood stasis syndrome group and healthy group were detected via high-throughput sequencing technology

and the differentially expressed genes related to CHD blood stasis syndrome were identified by using crosslinking analysis. Functional and pathway enrichment was performed for the obtained differentially expressed genes

and then the interaction network was constructed by Pearson correlation analysis and starBase prediction. Key nodes of interaction network were screened out by tropical analysis. Another cohort was enrolled to verify the key nodes in interaction network with Real-time PCR. Result: The 39 lncRNAs

229 miRNAs and 221 mRNAs were closely related to CHD blood stasis syndrome. Functional and pathway analysis results showed that the differentially expressed genes were majorly correlated to immune and inflammation. A total of 9 lncRNAs (all down-regulated)

31 mRNAs (11 up-regulated and 20 down-regulated) and 24 miRNAs (14 up-regulated and 10 down-regulated) constituted the regulation network

including 76 intergenic relationships. CTA-384D8.35

CTB-114C7.4

RP11-567M16.6 and hsa-miR-3158-3p were the key nodes in CHD blood stasis syndrome interaction network

which was verified by Real-time PCR. Conclusion: CHD blood stasis syndrome has specific lncRNA

miRNA and mRNA expression profiles

closed related to immunity and inflammation; gene-gene interactions exist among differentially expressed genes

and CHD blood stasis syndrome related lncRNA-miRNA-mRNA interaction network can be constructed on this basis.