Objective: To identify and determine the main chemical constituents in Lonicerae Japonicae Flos by RRLC-DAD-ESI-Q-TOF-MS

and establish the quantitative fingerprint of Lonicerae Japonicae Flos of Shandong province. Method: The rapid analysis of Lonicerae Japonicae Flos extract was carried out by RRLC-DAD-ESI-Q-TOF-MS. The chemical formula of Lonicerae Japonicae Flos was inferred by using the exact molecular weight and secondary mass spectrometry information. The chemical compositions of Lonicerae japonicae flos were identified by data base combined with literature reviewing. The contents of main chemical components in Lonicerae Japonicae Flos from Shandong and Henan as well as Lonicerae Flos were determined by using RRLC-DAD method

and the quantitative fingerprint was established on this basis for the quality evaluation of different samples. Result: The 51 chromatographic peaks were detected and 32 chemical constituents were identified from Lonicerae Japonicae Flos. The contents of 10 major chemical contents (loganic acid

chlorogenic acid

caffeic acid

loganin

secoxyloganin

rutin

hyperoside

luteoloside

isochlorogenic acid A and isochlorogenic acid C) in Lonicerae Japonicae Flos from Shandong and Henan as well as Lonicerae Flos were determined and compared. The differentiation of different species of Lonicerae Japonicae Flos and Lonicerae Flos was achieved by RRLC fingerprint combined with similarity analysis

and the result wasconsistent with the content determination result. Conclusion: RRLC-DAD-ESI-Q-TOF-MS technique is fast and accurate

providing accurate quality information for compounds and their fragment ions. It can quickly and accurately identify and determine the chemical compositions of Lonicerae Japonicae Flos

and the multiple indicator quantitative fingerprint based on this study could provide technology support for the quality evaluation of Lonicerae Japonicae Flos.