Objective: To establish the UPLC fingerprint of Chenxiang Huaqiwan for providing a reference for evaluation of the overall quality of this preparation. Method: The separation was performed on Phenomenex Kinetex C18 column (4.6 mm×100 mm

2.7 μm) with mobile phase of acetonitrile-0.1% phosphoric acid aqueous solution for gradient elution at a flow rate of 0.8 mL·min-1

wavelength switching technology was used (0-3 min

285 nm;3-20 min

210 nm;20-24 min

254 nm;24-42 min

210 nm).According to the similarity evaluation

the chemometrics method was used to evaluate the quality of Chenxiang Huaqiwan. Result: Fingerprints of 20 batches of Chenxiang Huaqiwan were established

there were 27 common peaks in the fingerprints and 12 common peaks were identified by reference substances

fingerprints similarity of samples were over 0.98.The samples were classified into three groups by hierarchical cluster analysis combined with principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA)

seven components were the main markers that cause differences in the different batches of samples. Conclusion: Establishment of fingerprint and application of chemical pattern recognition can provide more comprehensive reference for the quality control of Chenxiang Huaqiwan.The established UPLC fingerprint method is stable and feasible.