UPLC-MS考察西洋红参皂苷类成分对大鼠脑内神经化学物质的影响

黄鑫; 李帅坪; 张勇; 刘淑莹

doi:10.13422/j.cnki.syfjx.2017190111

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UPLC-MS考察西洋红参皂苷类成分对大鼠脑内神经化学物质的影响

药物代谢 | 更新时间：2024-01-04

- UPLC-MS考察西洋红参皂苷类成分对大鼠脑内神经化学物质的影响
- Effect of Saponins in Red Panacis Quinquefolii Radix on Neurochemicals in Rat Brain by UPLC-MS
- 中国实验方剂学杂志 2017年23卷第19期页码：111-117
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目（21475012）;吉林省科技发展计划项目（20160520181JH）;吉林省教育厅"十三五"科学技术研究项目（吉教科合字[2016]第30号）;首批长春中医药大学百名中青年骨干教师培养计划项目（2017086）
- DOI：10.13422/j.cnki.syfjx.2017190111
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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黄鑫, 李帅坪, 张勇, 等. UPLC-MS考察西洋红参皂苷类成分对大鼠脑内神经化学物质的影响[J]. 中国实验方剂学杂志, 2017,23(19):111-117.

HUANG Xin, LI Shuai-ping, ZHANG Yong, et al. Effect of Saponins in Red Panacis Quinquefolii Radix on Neurochemicals in Rat Brain by UPLC-MS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(19): 111-117.
黄鑫, 李帅坪, 张勇, 等. UPLC-MS考察西洋红参皂苷类成分对大鼠脑内神经化学物质的影响[J]. 中国实验方剂学杂志, 2017,23(19):111-117. DOI： 10.13422/j.cnki.syfjx.2017190111.

HUANG Xin, LI Shuai-ping, ZHANG Yong, et al. Effect of Saponins in Red Panacis Quinquefolii Radix on Neurochemicals in Rat Brain by UPLC-MS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(19): 111-117. DOI： 10.13422/j.cnki.syfjx.2017190111.

摘要

目的：考察西洋红参皂苷组分Ⅰ和Ⅱ对大鼠大脑皮层及海马中17种神经化学物质水平的影响。方法：将西洋红参总皂苷经D101型大孔吸附树脂分离纯化制备得到组分Ⅰ和Ⅱ，采用超高效液相色谱-线性离子阱质谱法（UPLC-LIT-MS）测定组分中皂苷单体的组成。采用超高效液相色谱-三重四级杆质谱法（UPLC-QQQ-MS）测定大鼠大脑皮层及海马中17种神经化学物质含量变化情况。结果：在与空白组比较，给予皂苷组分Ⅰ后，大鼠大脑皮层及海马中谷氨酸，天冬氨酸，牛磺酸，乙酰胆碱，酪氨酸，多巴胺，去甲肾上腺的含量升高，与给药剂量成正相关趋势；在给予皂苷组分Ⅱ后，γ-氨基丁酸，色氨酸，5-羟色胺，5-羟吲哚乙酸，褪黑素，丝氨酸，甘氨酸，组胺的含量升高，且与给药剂量成正相关趋势。通过分析皂苷组成与脑内神经化学物质含量变化之间的内在联系，发现组分Ⅰ可提高大脑皮层及海马中兴奋性神经化学物质含量，组分Ⅱ可提高大鼠大脑皮层及海马中抑制性神经化学物质含量。结论：西洋红参中皂苷组分具有神经活性，皂苷类成分的极性与其对兴奋性和抑制性神经化学物质的影响具有相关性。

Abstract

Objective: To investigate effects of saponin component Ⅰ and Ⅱ in red Panacis Quinquefolii Radix on 17 neurochemicals in rat cerebral cortex and hippocampus. Method: Saponin component Ⅰ and Ⅱ were prepared from total saponins of red Panacis Quinquefolii Radix by D101 macroporous adsorption resin.The composition of saponins in component Ⅰ and Ⅱ were detected by UPLC-LIT-MS.The contents of 17 neurochemicals in rat cerebral cortex and hippocampus were determined by UPLC-QQQ-MS. Result: Compared with the blank group

the contents of glutamic acid

aspartic acid

taurine

acetylcholine

tyrosine

dopamine and norepinephrine in rat cerebral cortex and hippocampus increased after administration of saponin component Ⅰ;while the contents of γ-aminobutyric acid

tryptophan

5-hydroxytryptamine

5-hydroxyindoleacetic acid

melatonin

serine

glycine and histamine increased after administration of saponin component Ⅱ;and the trends were positively related to dose.Through analyzing the relationship of saponin composition with content variation of neurochemicals

saponin component Ⅰ could increase the contents of excitatory neurochemicals in rat cerebral cortex and hippocampus

while saponin component Ⅱ were shown to improve the inhibitory neurochemicals contents in rat cerebral cortex and hippocampus. Conclusion: The saponins in red Panacis Quinquefolii Radix has neural activity.The polarity of saponins is related to their effects on excitatory and inhibitory neurochemicals.

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