开心散乙醇提取物中远志皂苷的入血成分及体内代谢产物分析

Reeju Maharjan; Chiranjivi Thapa; 汪娜; Ram Chaulagain; 薛迪; 贾永明; 邓竹君; 黄树明; 刘学伟

doi:10.13422/j.cnki.syfjx.2017190118

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开心散乙醇提取物中远志皂苷的入血成分及体内代谢产物分析

药物代谢 | 更新时间：2024-01-04

- 开心散乙醇提取物中远志皂苷的入血成分及体内代谢产物分析
- Identification of Polygala Saponins and Their Metabolites from Ethanol Extract of Kaixinsan
- 中国实验方剂学杂志 2017年23卷第19期页码：118-123
- 作者机构：
- 作者简介：
- 基金信息：
  
  黑龙江省教育厅基本科研业务费基础研究项目（2016-KYYWF-0865）
- DOI：10.13422/j.cnki.syfjx.2017190118
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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Reeju Maharjan, Chiranjivi Thapa, 汪娜, 等. 开心散乙醇提取物中远志皂苷的入血成分及体内代谢产物分析[J]. 中国实验方剂学杂志, 2017,23(19):118-123.

Reeju Maharjan, Chiranjivi Thapa, WANG Na, et al. Identification of Polygala Saponins and Their Metabolites from Ethanol Extract of Kaixinsan[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(19): 118-123.
Reeju Maharjan, Chiranjivi Thapa, 汪娜, 等. 开心散乙醇提取物中远志皂苷的入血成分及体内代谢产物分析[J]. 中国实验方剂学杂志, 2017,23(19):118-123. DOI： 10.13422/j.cnki.syfjx.2017190118.

Reeju Maharjan, Chiranjivi Thapa, WANG Na, et al. Identification of Polygala Saponins and Their Metabolites from Ethanol Extract of Kaixinsan[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(19): 118-123. DOI： 10.13422/j.cnki.syfjx.2017190118.

摘要

目的：鉴定开心散60%乙醇提取物（KXS-60% E）及其血中移行成分中的远志皂苷类成分，分析大鼠体内远志皂苷类成分的代谢产物。方法：应用UPLC-TOF-MS技术，分析TOF-MS/MS给出的二级离子信息，结合液质联用工作站MassLynx v4.1分析软件及现有的文献数据库，鉴定KXS-60% E中远志皂苷类成分、灌服KXS-60% E后血清中的远志皂苷类成分及其代谢产物。结果：鉴定了KXS-60% E中14个远志皂苷类成分，主要为三萜型皂苷；鉴定了大鼠口服KXS-60% E后血中的5个原型皂苷类成分及4个代谢产物。结论：远志皂苷类成分及4个代谢产物可能是KXS-60% E潜在的活性成分及直接药效物质基础，这将有助于阐明开心散防治阿尔兹海默症的有效成分及作用机制。

Abstract

Objective: Polygala saponins in the 60% ethanol extract of Kaixinsan (KXS-60% E) were identified

and the polygala saponins as well as their metabolites were analyzed in rat plasma after oral administration of KXS-60% E. Method: Ion fragments data of polygala saponins in KXS-60% E was identified by UPLC-TOF-MS.Similarly

the polygala saponins and their metabolites were also identified in rat plasma after oral administration of KXS-60% E with the ion fragments data

MassLynx v4.1 software and databases in the literatures. Result: A total of 14 polygala saponins which were mainly triterpene saponins

were identified in KXS-60% E in vitro.Moreover

5 polygala saponins as well as 4 metabolites were identified in rat plasma. Conclusion: Five polygala saponins as well as four metabolites may be the potential bioactive compounds and direct pharmcodynamic basis of KXS-60% E.Further study on these constituents may find out the effective compounds in KXS-60% E and clarify the mechanism on treatment of Alzheimer's disease.

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