LIU Rong-hua, WANG Ding-qing, LIN Shuai, et al. Triterpenoids from Heartwood of [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(20): 42-46.
LIU Rong-hua, WANG Ding-qing, LIN Shuai, et al. Triterpenoids from Heartwood of [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(20): 42-46. DOI: 10.13422/j.cnki.syfjx.2017200042.
Objective: To investigate the chemical constituents of triterpenoids in the heartwood of Dalbergia latifolia. Method: The heartwood samples of D.latifolia were crushed into powder and extracted with 70% ethanol for 3 times
2 h for each time.The filtrate liquid was collected and the solvent was decompressed and recovered to obtain extract.The extract was dispersed in water and then extracted successively with petroleum ester
dichloromethane
ethylacetate and butanol to obtain petroleum ester fraction
dichloromethane fraction
ethylacetate fraction and butanol fraction.The chemical compounds in the extract fractions were isolated by column chromatography technologies such as silica gel
Sephadex LH-20
reverse phase silica gel and macroporous resin
and the structures of the obtained monomeric compounds were identified through physical and chemical analysis
spectroscopic methods and mass spectrum analysis method. Result: Seven triterpenoids were isolated and identified as 3-O-α-L-arabinopyranosyl-28-O-β-D-glucopyranosyl pomolic acid (1)
β-sitostenone (2)
β-sitosterol (3)
stigmasterol (4)
β-amyrin acetate (5)
β-amyrin-3-palmitate (6)
and 3-acetoxy-oleanoic acid (7). Conclusion: Compounds 1
5
and 6 were isolated from Dalbergia genus for the first time.Compounds 2 and 4 were isolated from this plant for the first time.