Objective: To simultaneously determinate the contents of protocatechuic acid

chlorogenic acid

hyperoside

specnuezhenide

epmedin C and icariin in Bushen Qiangshen pills (BQP) by developing an Ultra high performance liquid method(UPLC). Method: The UPLC separation was performed on an Agilent SB-C18 reversed-phase column (4.6 mm×100 mm

2.7 μm) with acetonitrile-0.1% phosphoric acid solution as the mobile phase for gradient elution.The detective wavelength was set at 260

325

360

224

270 nm. Result: The calibration curve was linear when amount was within 0.78-201.00 mg ·L-1 for protocatechuic acid (r=0.999 9); 0.79-202.60 mg · L-1 for chlorogenic acid (r=0.999 7); 0.93-238.39 mg ·L-1 for hyperoside (r=0.999 9); 0.66-169.62 mg ·L-1 for specnuezhenide (r=0.999 9); 0.81-206.85 mg ·L-1 for epmedin C (r=0.999 9); and 0.74-190.85 mg ·L-1for icariin (r=0.999 9).The recoveries were 97.6%

99.6%

99.7%

97.2%

95.5%

96.3% with RSDs of 1.0%

1.5%

1.5%

0.8%

0.4% and 0.9%

respectively. Conclusion: This simple and reliable method can be used for the quality control of BQP.