Objective: To establish a method for simultaneously determining the contents of puerarin

thymol

carvacrol

honokiol

hesperidin and daidzin in Jiawei Xiangru oral liquid (Puerariae Lobatae Radix

Moslae Herba

Magnoliae Officinalis Cortex

Citri Reticulatae Pericarpium

Bombyx Mori Linnacus and Lablab Semen Alabum). Method: High performance liquid chromatography (HPLC) was performed on Hypersil C18 chromatographic column (4.6 mm×250 mm

5 μm) with mobile phase of acetonitrile-0.01% glacial acetic acid (gradient elution) at a flow rate of 1 mL · min-1.The detction wavelength was 277 nm

and the column temperature was 30℃. Result: Puerarin

thymol

carvacrol

honokiol

hesperidin and daidzin showed good linear relationship between injection volume and peak area within the range of 0.384-4.800 μg (r=0.999 3)

0.048-0.600 μg (r=0.999 7)

0.015 6-0.195 0 μg (r=0.999 8)

0.018 2-0.227 5 μg (r=0.999 2)

0.226-2.825 μg (r=0.999 6)

and 0.228-2.850 μg (r=0.999 5)

respectively.The average recovery rate was 97.98%

98.88%

98.10%

100.12%

100.40% and 96.70%

respectively for puerarin

thymol

carvacrol

honokiol

hesperidin and daidzin

with RSDs of 2.1%

1.8%

2.1%

1.5%

2.6%

and 1.6%. Conclusion: The method is simple

stable and reproducible

and it can be used for the simultaneous determination of puerarin

thymol

carvacrol

honokiol

hesperidin and daidzin contents in Jiawei Xiangru oral liquid.