Objective: To explore the regulating effect of Yishen Jiedu prescription on the protein expression of ribonuclease L (RNase L) and the apoptosis terminal protein Caspase-3 in bone metastasis from lung adenocarcinoma. Method: Lung adenocarcinoma cell lines that had won the national patent were injected into left ventricle of nude mice to prepare the bone metastasis model.Then

the mice were randomly divided into five groups:high

middle and low-dose (24

12

3 g · kg-1) Yishen Jiedu groups

disodium phosphate group and control group.Finally

with histopathological method and microSPECT/CT imaging method

we can observe the outcome of bone metastases.After administration at fixed time and dose for 5 weeks

dissection was performed

and the expressions of RNase L protein and Caspase-3 protein in bone metastasis from lung adenocarcinoma

and the regulatory effect of Yishen Jiedu prescription was analyzed through immunohistochemistry methods

Western blot and reverse transcription polymerase chain reaction (RT-PCR) methods. Result: The model group had the highest expression of RNase L protein and mRNA in bone metastases tumor cells

with significant statistical differences between the model group and others (P<0.01).However

the model group showed the lowest expression of Caspase-3 protein and mRNA

with significant statistical differences between the middle low dosage group of Yishen Jiedu and disodium phosphate groups (P<0.01). Conclusion: There was a high expression in RNase L expression in bone metastases in patients with lung adenocarcinoma

while Caspase-3

a main terminal protein in the process of cell apoptosis

was extremely low.The internal mechanism may be correlated with the cell apoptosis escape of lung adenocarcinoma bone metastases.The prescription of Yishen Jiedu prescription can inhibit the apoptosis of tumor escape by reducing the expression of RNase L and enhancing caspase-3 protein.