Objective: To compare the effect of herb pair of Rhei Radix et Rhizoma and Salviae Miltiorrhizae Radix et Rhizoma with different medicinal guides on hepatic fibrosis rats. Method: Sixty SD rats were randomly divided into normal group

model group

herb pair group (5.0 g · kg-1)

herb pair plus Platycodonis Radix (5.0 g · kg-1+1.5 g · kg-1)

herb pair plus Bupleuri Radix group (5.0 g · kg-1+1.5 g · kg-1) and herb pair plus Asari Radix et Rhizom group (5.0 g · kg-1+0.3 g · kg-1). The hepatic fibrosis rat models were induced through intraperitoneal injection with CCl4.Since the 3rd week after modeling

different drugs were administrated by gavage for 6 weeks. Rats were put to death after the last administration

and their serum was collected to measure activities of serum alanin aminotransferase (ALT)

aspartate aminotransferase (AST) and alkaline phosphatase (ALP). Levels of laminin (LN)

hyaluronic acid (HA) and procollagen Ⅲ (PCⅢ) in serum were detected by enzyme linked immunosorbent assay (ELISA). The content of hydroxyproline (HYP) in liver homogenate was measured by acid hydrolysis method. The expression of transforming growth factor-β1 (TGF-β1) in liver was analyzed by immunohistochemical method. Result: Compared with the model group

the levels of ALT

AST

ALP

LN

HA

PCⅢ and the content of HYP in experimental groups were significantly decreased

the expression of TGF-β1 in liver was reduced (P<0.05

P<0.01)

the liver pathological fibrosis was obviously relieved. Compared with the herb pair group

the levels of ALT

AST

ALP

LN

HA

PCⅢ and the expression of TGF-β1 in the herb pair plus Bupleuri Radix group were significantly decreased (P<0.05

P<0.01). Conclusion: The herb pair of Rhei Radix et Rhizoma and Salviae Miltiorrhizae Radix et Rhizoma has a protective effect on liver fibrosis rat

and Bupleuri Radix could further improve the curative effect.