哈蟆油蛋白对骨质疏松预防及ALP,Osteocalcin,Runx-2基因表达的调节作用

李晓华; 陈锡俊; 李娜; 石晓征; 韩冬; 黄晓巍; 曲晓波

doi:10.13422/j.cnki.syfjx.2017200138

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哈蟆油蛋白对骨质疏松预防及ALP,Osteocalcin,Runx-2基因表达的调节作用

药理 | 更新时间：2024-01-04

- 哈蟆油蛋白对骨质疏松预防及ALP,Osteocalcin,Runx-2基因表达的调节作用
- Effect of Ranae Oviductus Protein in Preventing Osteoporosis and Regulating ALP, Osteocalcin and Runx-2 Gene Expression
- 中国实验方剂学杂志 2017年23卷第20期页码：138-143
- 作者机构：
- 作者简介：
- 基金信息：
  
  吉林省科技发展计划项目（20150309008YY）;吉林省中医药科技项目（2017002）
- DOI：10.13422/j.cnki.syfjx.2017200138
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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李晓华, 陈锡俊, 李娜, 等. 哈蟆油蛋白对骨质疏松预防及ALP,Osteocalcin,Runx-2基因表达的调节作用[J]. 中国实验方剂学杂志, 2017,23(20):138-143.

LI Xiao-hua, CHEN Xi-jun, LI Na, et al. Effect of Ranae Oviductus Protein in Preventing Osteoporosis and Regulating ALP, Osteocalcin and Runx-2 Gene Expression[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(20): 138-143.
李晓华, 陈锡俊, 李娜, 等. 哈蟆油蛋白对骨质疏松预防及ALP,Osteocalcin,Runx-2基因表达的调节作用[J]. 中国实验方剂学杂志, 2017,23(20):138-143. DOI： 10.13422/j.cnki.syfjx.2017200138.

LI Xiao-hua, CHEN Xi-jun, LI Na, et al. Effect of Ranae Oviductus Protein in Preventing Osteoporosis and Regulating ALP, Osteocalcin and Runx-2 Gene Expression[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(20): 138-143. DOI： 10.13422/j.cnki.syfjx.2017200138.

摘要

目的：观察哈蟆油蛋白（ROP）的抗骨质疏松作用，并探讨其对骨生长相关基因的调节作用。方法：采用去势法建立大鼠骨质疏松模型，随机分为空白组，模型组，戊酸雌二醇组，ROP高、中、低剂量组（0.15，0.075，0.037 5 g ·kg-1）；利用X射线技术测量股骨和腰椎骨密度（BMD）；利用骨骼强度仪检测股骨的最大载荷量；应用实时荧光定量PCR（Real-time PCR）技术检测骨生长相关基因的表达。结果：与空白组比较，模型组股骨、腰椎骨密度明显降低（P<0.05，P<0.01），碱性磷酸酶（alkaline phosphatase，ALP），Osteocalcin，Runx-2基因表达均显著下调（P<0.01）；与模型组比较，ROP高、中剂量组腰椎BMD均有显著增加（P<0.01），ROP高剂量组ALP，Osteocalcin，Runx-2基因表达显著上调（P<0.01）。结论： ROP能显著提高大鼠BMD和最大载荷量；ROP可能通过上调ALP，Osteocalcin，Runx-2基因的mRNA表达，促进成骨分化，调节骨代谢平衡，从而发挥防治骨质疏松的作用。

Abstract

Objective: To observe the anti-osteoporosis effect of Ranae Oviductus protein (ROP)

and the regulatory effect on bone growth related genes. Method: The osteoporosis model of rats were established by castration method

and randomly divided into 6 groups

namely blank group

model group

Estradiol valerate group

and high-does

medium-does and low-does (0.15

0.075

0.037 5 g ·kg-1) ROP groups.The bone mineral densities of femur and lumbar were measured by X-ray technique. The maximum load of femur was measured by bone strength instrument.Quantitative analysis was made for the expressions of key genes of bone growth by Real-time PCR. Result: Compared with the blank group

the BMD of femur and lumbar in the model group decreased significantly (P<0.05

P<0.01)

the expressions of alkaline phosphatase (ALP)

Osteocalcin and Runx-2 gene decreased significantly (P<0.01).Compared with the model group

the BMD of lumbar vertebrae in high and middle-dose groups increased significantly (P<0.01)

the expressions of ALP

Osteocalcin and Runx-2 gene in the high-dose group were significantly increased (P<0.01). Conclusion: ROP can significantly improve bone mineral density and maximum load in rats

improve the mRNA expressions of ALP

Osteocalcin and Runx-2 gene

promote the osteoblast differentiation

and regulate the bone substitute balance

so as to play a role in preventing and treating osteoporosis.

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