Objective: To observe the effect of anisodine hydromide on the expression of autophagy-related protein

in order to explore the protective effect and possible mechanism of anisodine hydromide on rats pheochromocytoma cells (PC12) induced by hypoxia. Method: PC12 cells were divided into control group

model group

n-butylphthalide group

and high-dose

middle-dose and low-dose anisodine hydromide groups (100

50

25 μmol · L-1).The relative viability of PC12 cells induced by hypoxia was measured by thiazole blue chromatomentry (MTT)

and the morphology of PC12 cells was observed.The morphological changes in autophagosome were observed by transmission electron microscope and monodansylcadaverine (MDC) fluorescence staining.The expressions of microtubule associated protein 1 light chain3 (LC3) and Beclin-1 protein were detected by Western blot method. Result: The viability rates of cells were significantly improved after intervention with anisodine hydromide

and the expressions of LC3-Ⅱ and Beclin-1 were significantly improved after intervention with anisodine hydromide.These results were consistent with the observation of transmission electronmicroscope and MDC fluorescence staining. Conclusion: Autophagy has a protective effect on PC12 cell induced by hypoxia

and anisodine hydromide could prevent the injury of PC12 cells induced by hypoxia through activating autophagy.