Objective: To establish a method for the content determination of aceglutamide

hydroxysafflower yellow A

rutin

syringing

kaempferide

kaempferol and quercetin in Guhong injection. Method: UPLC-MS/MS was conducted. The column of UPLC was Phenomenex Kenetix C18 (2.1 mm×50 mm

5 μm)

with mobile phase of acetonitrile-0.1% formic acid (gradient elution) at a flow of 0.5 mL·min-1. The multiple reaction monitoring mode (MRM) was adopted with simultaneous monitoring of positive and negative ions and

the injection volume was 5 μL. Result: The linear range was 10.36-663.0 μg·L-1 for aceglutamide

0.500 0-32.00 μg·L-1 for hydroxysafflower yellow A

0.047 8-3.059 μg·L-1 for rutin

0.200 0-12.80 μg·L-1for syringing

0.032 4-2.074 μg·L-1for kaempferide

0.675 0-43.20 μg·L-1 for kaempferol

and 0.142 5-9.120 μg·L-1 for quercetin. The recoveries were between 97.0% and 99.2% (RSD ≤ 3.4%

n=6). The contents of aceglutamide

hydroxysafflower yellow A

rutin

syringing

kaempferide

kaempferol

quercetin in the three batches of samples ranged between 28.5-32.4 g·L-1

1.02-1.23 g·L-1

14.3-17.6 mg·L-1

101-123 mg·L-1

12.1-14.9 μg·L-1

0.138-0.155 mg·L-1

73.4-95.6 μg·L-1. Conclusion: The method is convenient

rapid

effective

simple

accurate

reliable and suitable for the simultaneous determination for 7 ingredients in Guhong injection and offers a basis for establishing a more comprehensive quantity control method.