Objective: To apply GC calibration factors in quantitative analysis of safrole and methyl eugenol in Asarum. Method: First

Agilent 6890N GC

detector FID

column DB-WAX (250 μm×30 m

0.25 μm) were used with detector temperature of 250 ℃

inlet temperature of 230 ℃

gas of N2 under constant pressure mode. The flow rate was 1.0 mL·min-1

injection volume of 1 μL

hydrogen flow of 45 mL·min-1

air flow of 450 mL·min-1

and tail blown nitrogen of 35 mL·min-1. The initial temperature was 135 ℃ for 2 min; rose to 160 ℃ at 5 ℃·min-1 and maintained for 7 min; then rose to 220 ℃ at 20 ℃·min-1 and maintained for 3 min. After that

a variety of determination and calculation methods were adopted to obtain a number of GC calibration factor values. Finally

after verification of their feasibility through stability

repeatability and sample recovery rate tests to screen the calibration factors for quantitative analysis of safrole and methyl eugenol. Result: The values calculated in stability and repeatability tests all had RSDs less than 3%; the average recoveries were 92.96%-121.35%

with RSDs of 3.4%-4.8% for safrole; and 88.80%-112.54% with RSDs of 2.0%-3.4% for methyl eugenol. Finally

10 batches of Asarum of safrole and methyl eugenol were determined by two GC calibration factors. Conclusion: Two GC correction factor values did not have many differences for quantitative analysis of safrole and methyl eugenol in Asarum

and the application of various determination and calculation methods of GC calibration factors can improve the accuracy of the test results.