甘肃红芪和黄芪总多糖含量测定对比

王瑞海; 叶迎; 许京; 苗青; 柏冬; 刘丽梅

doi:10.13422/j.cnki.syfjx.2017210077

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甘肃红芪和黄芪总多糖含量测定对比

化学与分析 | 更新时间：2024-01-04

- 甘肃红芪和黄芪总多糖含量测定对比
- Total Polysaccharides Content Determination of Hedysari Radix and Astragali Radix from Gansu Province
- 中国实验方剂学杂志 2017年23卷第22期页码：77-83
- 作者机构：
- 作者简介：
- 基金信息：
  
  中国中医科学院基本科研业务费自主选题项目（YZ-1438）。
- DOI：10.13422/j.cnki.syfjx.2017210077
  中图分类号： R284.1
- 纸质出版日期：2017
- 稿件说明：
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王瑞海, 叶迎, 许京, 等. 甘肃红芪和黄芪总多糖含量测定对比[J]. 中国实验方剂学杂志, 2017,23(22):77-83.

WANG Rui-hai, YE Ying, XU Jing, et al. Total Polysaccharides Content Determination of Hedysari Radix and Astragali Radix from Gansu Province[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(22): 77-83.
王瑞海, 叶迎, 许京, 等. 甘肃红芪和黄芪总多糖含量测定对比[J]. 中国实验方剂学杂志, 2017,23(22):77-83. DOI： 10.13422/j.cnki.syfjx.2017210077.

WANG Rui-hai, YE Ying, XU Jing, et al. Total Polysaccharides Content Determination of Hedysari Radix and Astragali Radix from Gansu Province[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(22): 77-83. DOI： 10.13422/j.cnki.syfjx.2017210077.

摘要

目的：建立甘肃不同产地红芪和黄芪药材中总多糖含量测定的方法，比较不同产地二者总多糖含量，以及不同生长年限（1，2年）对2种药材总多糖含量的影响。方法：以葡萄糖为对照品，采用苯酚-硫酸显色法，在490 nm波长处测定甘肃不同产地红芪和黄芪共28批样品中总多糖含量。结果：葡萄糖在16.5~165 μg与吸光度线性关系良好（r=0.999 9），红芪和黄芪平均加样回收率分别为97.43%和100.33%，RSD分别为2.1%和2.3%（n=6）。1年生红芪中总多糖平均质量分数分别为宕昌地区11.45%，武都地区5.96%，陇西地区10.97%，甘肃各地区总平均9.35%；黄芪中总多糖平均质量分数分别为宕昌地区3.69%，武都地区1.93%，陇西地区7.06%，甘肃各地区总平均4.02%；2年生红芪样品中总多糖平均质量分数分别为宕昌地区6.28%，武都地区5.82%，陇西地区6.69%，甘肃各地区总平均6.23%；黄芪中总多糖平均质量分数分别为宕昌地区7.91%，武都地区8.03%，陇西地区7.12%，甘肃各地区总平均7.73%。结论：建立的测定方法简便，准确，重复性好，可用于样品中总多糖含量测定。甘肃省5县，1市区红芪和黄芪在品种及年限对比中均有极显著性差异（P<0.01），从多糖含量分析，红芪不适合替代黄芪使用。各地1年生红芪中总多糖含量均高于黄芪，2年生红芪总多糖含量均低于黄芪。红芪以宕昌产较优，且以1年生为最好；黄芪1年生以陇西、岷县较优，2年生以武都较优。

Abstract

Objective: To establish a method to determine the total polysaccharides content in Hedysari Radix and Astragali Radix from different areas of Gansu province

compare their contents of total polysaccharides from different habitats

and explore the effect of different growth years (1

2 years) on the total polysaccharides content. Method: Total polysaccharides of Hedysari Radix and Astragali Radix from different areas of Gansu province were determined by using the phenol sulfuric acid method at 490 nm

with glucose as reference substance. Result: A good linear relationship of glucose was achieved within the range of 16.5-165 μg (r=0.999 9)

the average recoveries of Hedysari Radix and Astragali Radix were 97.43% (RSD 2.1%

n=6) and 100.33% (RSD 2.3%

n=6).For one-year-old plant

the average content of total polysaccharides in Hedysari Radix from Dangchang

Wudu

Longxi and Gansu were 11.45%

5.96%

10.97% and 9.35%

respectively. The average contents of total polysaccharides in Astragali Radix from Dangchang

Wudu

Longxi and and Gansu were 3.69%

1.93%

7.06% and 4.02%

respectively. For two-year-old plant

the average contents of total polysaccharides in Hedysari Radix from Dangchang

Wudu

Longxi and Gansu were 6.28%

5.82%

6.69% and 6.23%

respectively. The average contents of total polysaccharides in Astragali Radix from Dangchang

Wudu

Longxi and Gansu were 7.91%

8.03%

7.12% and 7.73%

respectively. Conclusion: The method is simple

accurate and reproducible

and can be used for the determination of total polysaccharides in Hedysari Radix and Astragali Radix. There was significant differences in varieties and years between the content of total polysaccharides in Hedysari Radix and Astragali Radix from 5 counties and 1 city in Gansu province (P<0.01). According to the analysis of the content of total polysaccharides

Hedysari Radix was not suitable to substitute Astragali Radix. The total polysaccharide content of one-year-old Hedysari Radix was higher than that of Astragali Radix

and the result of two-year-old plants was lower than that of Astragali Radix. Hedysari Radix in Dangchang is better

and the one-year-old Hedysari Radix is the best. One-year-old Astragali Radix in Longxi

Minxian is better

and two-year-old Astragali Radix in Wudu is better.

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