白头翁皂苷B4在正常大鼠和溃疡性结肠炎大鼠体内组织分布情况的比较

杨连荣; 张丽杰; 姚姣姣; 张凌; 刘亚丽; 范卓文

doi:10.13422/j.cnki.syfjx.2017210093

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白头翁皂苷B4在正常大鼠和溃疡性结肠炎大鼠体内组织分布情况的比较

药物代谢 | 更新时间：2024-01-04

- 白头翁皂苷B4在正常大鼠和溃疡性结肠炎大鼠体内组织分布情况的比较
- Comparison of Tissue Distribution Profiles of Anemoside B4 After Intragastric Administration of Effective Part of Baitouweng Tang in Normal and Ulcerative Colitis Rats
- 中国实验方剂学杂志 2017年23卷第21期页码：93-97
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81603377）
- DOI：10.13422/j.cnki.syfjx.2017210093
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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杨连荣, 张丽杰, 姚姣姣, 等. 白头翁皂苷B4在正常大鼠和溃疡性结肠炎大鼠体内组织分布情况的比较[J]. 中国实验方剂学杂志, 2017,23(21):93-97.

YANG Lian-rong, ZHANG Li-jie, YAO Jiao-jiao, et al. Comparison of Tissue Distribution Profiles of Anemoside B4 After Intragastric Administration of Effective Part of Baitouweng Tang in Normal and Ulcerative Colitis Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(21): 93-97.
杨连荣, 张丽杰, 姚姣姣, 等. 白头翁皂苷B4在正常大鼠和溃疡性结肠炎大鼠体内组织分布情况的比较[J]. 中国实验方剂学杂志, 2017,23(21):93-97. DOI： 10.13422/j.cnki.syfjx.2017210093.

YANG Lian-rong, ZHANG Li-jie, YAO Jiao-jiao, et al. Comparison of Tissue Distribution Profiles of Anemoside B4 After Intragastric Administration of Effective Part of Baitouweng Tang in Normal and Ulcerative Colitis Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(21): 93-97. DOI： 10.13422/j.cnki.syfjx.2017210093.

摘要

目的：建立大鼠体内白头翁皂苷B4的UPLC-ESI-MS/MS，比较白头翁皂苷B4在正常大鼠和溃疡性结肠炎大鼠肝、脾、肾、心、肺组织中的分布情况，为研究白头翁汤治疗溃疡性结肠炎的作用过程和机制提供参考。方法：雄性SD大鼠等分为正常组和模型组，均按5 g·kg-1灌胃给药白头翁汤冻干粉，分别于15，45，120，360 min用5%水合氯醛麻醉正常大鼠和模型大鼠，取肝、脾、肾、心、肺组织，采用UPLC-ESI-MS/MS分析，以0.1%甲酸水溶液和0.1%甲酸乙腈溶液为流动相进行梯度洗脱，ACQUITY BEH C18色谱柱（2.1 mm×50 mm，1.7 μm），柱温35 ℃，流速0.4 mL·min-1。结果：白头翁皂苷B4在大鼠体内存在广泛分布与代谢。在正常大鼠的肝、脾、肾、心、肺组织中，白头翁皂苷B4均在120 min时达到最大质量浓度。与正常组比较，在模型组大鼠肝、心组织中白头翁皂苷B4质量浓度各时间点均明显升高（P < 0.05）；在脾和肾组织中，分别于45 min和120 min时明显升高（P < 0.05）；在肺组织中，于120 min时显著增加（P < 0.05）。结论：溃疡性结肠炎大鼠对白头翁皂苷B4的吸收和生物利用度均优于正常组，溃疡性结肠炎的病理状态可导致白头翁汤中白头翁皂苷B4的吸收增强。

Abstract

Objective: To establish a UPLC-ESI-MS/MS for determining anemoside B4 in rats

through comparing the distribution of anemoside B4 in liver

spleen

kidney

heart and lung of normal rats and rats with ulcerative colitis

in order to further study on the process and mechanism of Baitouweng Tang in treating ulcerative colitis. Method: Male SD rats were divided into normal group and model group

after intragastric administration of Baitouweng Tang lyophilized powder with dose of 5 g·kg-1

normal and model rats were anesthetized with 5% chloral hydrate at 15

120

360 min

then to take liver

spleen

kidney

heart and lung tissue.UPLC-ESI-MS/MS was adopted with mobile phase of 0.1% formic acid aqueous solution and 0.1% formic acid acetonitrile solution for gradient elution

column temperature of 35 ℃ and flow rate of 0.4 mL·min-1. Result: Anemoside B4 was widely distributed and metabolized in rats.The concentration of anemoside B4 in liver

spleen

kidney

heart and lung of normal rats reached the maximum at 120 min.Compared with the normal group

the content of anemoside B4 in liver and heart of model group rats was significantly increased (P < 0.05) at each time point;in spleen and kidney

it increased significantly (P < 0.05) at 45 min and 120 min;in the lung

it increased significantly (P < 0.05) at 120 min. Conclusion: Absorption and bioavailability of anemoside B4 in ulcerative colitis rats are better than those of the normal group

and the pathological state of ulcerative colitis can cause absorption enhancement of anemoside B4 in Baitouweng Tang.

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