狼毒大戟配伍大枣对诱导肝硬化大鼠的抵抗作用及机制

李文静; 兴桂华; 刘军; 徐天娇; 范松婕; 王玉春; 马春梅; 洪博

doi:10.13422/j.cnki.syfjx.2017210117

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狼毒大戟配伍大枣对诱导肝硬化大鼠的抵抗作用及机制

药理 | 更新时间：2024-01-04

- 狼毒大戟配伍大枣对诱导肝硬化大鼠的抵抗作用及机制
- Resistance Effect and Mechanism of Radix of Combined with Jujubae Fructus on Cirrhosis in Rats
- 中国实验方剂学杂志 2017年23卷第21期页码：117-123
- 作者机构：
- 作者简介：
- 基金信息：
  
  黑龙江省普通本科高等学校青年创新人才培养计划项目（7UNPYSCT-201611）
- DOI：10.13422/j.cnki.syfjx.2017210117
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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李文静, 兴桂华, 刘军, 等. 狼毒大戟配伍大枣对诱导肝硬化大鼠的抵抗作用及机制[J]. 中国实验方剂学杂志, 2017,23(21):117-123.

LI Wen-jing, XING Gui-hua, LIU Jun, et al. Resistance Effect and Mechanism of Radix of Combined with Jujubae Fructus on Cirrhosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(21): 117-123.
李文静, 兴桂华, 刘军, 等. 狼毒大戟配伍大枣对诱导肝硬化大鼠的抵抗作用及机制[J]. 中国实验方剂学杂志, 2017,23(21):117-123. DOI： 10.13422/j.cnki.syfjx.2017210117.

LI Wen-jing, XING Gui-hua, LIU Jun, et al. Resistance Effect and Mechanism of Radix of Combined with Jujubae Fructus on Cirrhosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(21): 117-123. DOI： 10.13422/j.cnki.syfjx.2017210117.

摘要

目的：研究狼毒大戟配伍大枣对肝硬化腹水大鼠模型的抵抗作用。方法：采用苯巴比妥联合四氯化碳（CCl4）法建立肝硬化腹水大鼠模型，将大鼠随机分为正常组、肝硬化腹水模型组、狼毒大戟组，狼毒大戟配伍大枣组，每组各10只。观测大鼠水代谢及苏木素-伊红（HE）染色观察肝组织病理变化，用免疫组织化学法测定I型胶原（Collagen Ⅰ），Ⅲ型胶原（Collagen Ⅲ），纤维粘连蛋白（Fibronectin，FN），层粘连蛋白（Laminin，LN），结蛋白（Desmin）在肝组织中的表达。结果：与模型组比较，狼毒大戟组及狼毒大戟配伍大枣组大鼠24 h尿量明显增多（P < 0.01），腹腔积液显著减少（P < 0.01）。给狼毒大戟配伍大枣组大鼠肝脏纤维间隔及变性程度较模型组有明显改善。免疫组化结果显示，肝硬化模型组及狼毒大戟治疗组，Ⅰ，Ⅲ型胶原及纤维连接蛋白的含量均显著高于正常组（P < 0.01），模型组LN和Desmin的含量显著高于正常组（P < 0.01），狼毒大戟配伍大枣组能显著降低LN和Desmin的积分吸光度IA。结论：狼毒大戟配伍大枣可明显改善肝硬化腹水大鼠肝硬化病理进程：增加尿量，促进腹水排泄，其作用机制可能与狼毒大戟配伍大枣对Collagen Ⅰ，Collagen Ⅲ，FN，LN，Desmin的调控有关。

Abstract

Objective: To research the resistance effect of radix of Euphorbia fisheriana(REF) combined with Jujubae Fructus(JF) on liver cirrhosis ascites in rat model. Method: Phenobarbital combined with CCl4 method was used to establish the rat live cirrhosis ascites model.The rats were divided into 4 groups

namely normal group

model group

REF group

and REF combined with JF group

with 10 rats in each group.Water metabolism and liver pathological changes in 4 groups were observed by HE staining method.Expressions of Collagen Ⅰ

Collagen Ⅲ

Fibronectin (FN)

Laminin

Desmin in different groups of rat liver issues were determined by immunohistochemical method. Result: Compared with the model group

the REF group and the REF combined with JF group significantly increase 24 h urine volume (P < 0.01)

and reduced peritoneal seroperitoneum (P < 0.01). Compared with the model group

the treatment groups significantly improved liver fiber interval and degeneration degree.The results of immunohistochemistry showed that the contents of Collagen Ⅰ

Collagen Ⅲ and FN in liver cirrhosis model group and REF group were significantly higher than those in the normal group (P < 0.01).The contents of LN and Desmin in the model group wree significantly higher than those in the normal group (P < 0.01)

and the treatment group can significantly decreased integral absorbance (IA). Conclusion: The combination of REF and JF can obviously improve the pathological process of cirrhotic ascites in rats

increase the amount of urine

and promote the excretion of ascites.The mechanism may be related to the regulatory effect of REF combined with JF on Collagen Ⅰ

Collagen Ⅲ

Desmin factors.

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