葛根芩连汤对2型糖尿病ZDF大鼠CRP，TNF-，IL-6的影响

王烨; 朱向东

doi:10.13422/j.cnki.syfjx.2017210130

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葛根芩连汤对2型糖尿病ZDF大鼠CRP，TNF-，IL-6的影响

药理 | 更新时间：2024-01-04

- 葛根芩连汤对2型糖尿病ZDF大鼠CRP，TNF-，IL-6的影响
- Effect of Gegen Qinlian Tang on CRP, TNF-alpha and IL-6 in Diabetic ZDF Rats
- 中国实验方剂学杂志 2017年23卷第21期页码：130-134
- 作者机构：
- 作者简介：
- 基金信息：
  
  中国博士后科学基金一等项目（2014M560168）
- DOI：10.13422/j.cnki.syfjx.2017210130
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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王烨, 朱向东. 葛根芩连汤对2型糖尿病ZDF大鼠CRP，TNF-，IL-6的影响[J]. 中国实验方剂学杂志, 2017,23(21):130-134.

WANG Ye, ZHU Xiang-dong. Effect of Gegen Qinlian Tang on CRP, TNF-alpha and IL-6 in Diabetic ZDF Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(21): 130-134.
王烨, 朱向东. 葛根芩连汤对2型糖尿病ZDF大鼠CRP，TNF-，IL-6的影响[J]. 中国实验方剂学杂志, 2017,23(21):130-134. DOI： 10.13422/j.cnki.syfjx.2017210130.

WANG Ye, ZHU Xiang-dong. Effect of Gegen Qinlian Tang on CRP, TNF-alpha and IL-6 in Diabetic ZDF Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(21): 130-134. DOI： 10.13422/j.cnki.syfjx.2017210130.

摘要

目的：研究葛根芩连汤（Gegen Qinlian Tang，GQT）对自发型2型糖尿病（type 2 diabetes mellitus，T2DM）肥胖大鼠（Zucker diabetes fatty，ZDF）中相关细胞因子C反应蛋白（C reactive protein，CRP），肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α），白细胞介素-6（interleukin-6，IL-6）表达的影响。方法：8只雄性ZDF（fa/+）大鼠设为正常组，成模的雄性ZDF（fa/fa）大鼠24只随机分为模型组（生理盐水10 mL·kg-1），二甲双胍（300 mg·kg-1）组，葛根芩连汤（生药量 13.8 g·kg-1）组。每2周测大鼠体重、空腹血糖（fasting blood-glucose，FBG），给药8周后取材，全自动生化法检测甘油三酯（triglyceride，TG），总胆固醇（total cholesterol，TC）；酶联免疫吸附法（enzyme linked immunosorbent assay，ELISA）检测大鼠血清中CRP，空腹胰岛素（fasting insulin，FINS）的量；计算胰岛素抵抗指数（insulin resistance index，IRI），胰岛素敏感指数（insulin sensitivity index，ISI）；蛋白免疫印迹法（Western blot）检测TNF-α，IL-6的表达。结果：与模型组比较，葛根芩连汤组体重，FBG，TG，TC，CRP，FINS，IRI，ISI明显改善（P < 0.05），葛根芩连汤组TNF-α，IL-6相对表达显著降低（P < 0.05）。结论：葛根芩连汤能有效改善2型糖尿病ZDF大鼠胰岛素抵抗，其作用机制可能与相关低度炎症反应中细胞因子的表达有关。

Abstract

Objective: To investigate the effects of Gegen Qinlian Tang (GQT) on expressions of inflammatory cytokines C reactive protein (CRP)

tumor necrosis factor-α (TNF-α)

interleukin-6 (IL-6) of Zucker diabetes fatty (ZDF) rats with type 2 diabetes mellitus (T2DM). Method: The 8 male ZDF(fa/+) rats were used as normal group

24 male ZDF rats (fa/fa) rats were randomized divided into model group (Nacl

10 mL·kg-1)

Metformin group (300 mg·kg-1)

GQT group (13.8 g·kg-1); and Zucker lean rats (ZL) were included into the blank control group

with 8 rats in each group. Weight and fasting blood-glucose(FBG) of rats were observed once every two weeks. Samples were taken 8 weeks after administration. Automatic biochemical analyzer was used to triglyceride(TG) and total cholesterol(TC). Enzyme linked immunosorbent assay(ELISA) was used to detect CRP and fasting insulin in rat serum(FINS)

and the expressions of TNF-α and IL-6 was detected by Western blot. Result: Compared with model group

GQT group show significant improvements in weight

FBG

CRP

FINS

IRI and ISI (P < 0.05). Meanwhile

GQT group can reduce the expressions of CRP

TNF-α and IL-6 in obesity type T2DM rats (P < 0.05). Conclusion: GQT can effectively reduce insulin resistance among ZDF rats with T2DM. Its action mechanism may be correlated with decrease in expressions of cytokines in relevant low-grade inflammatory reactions.

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