白马骨水提物对急性肝损伤小鼠氧化应激及炎症反应的影响

高雅; 王刚; 杜沛霖; 陈春; 曹后康; 葸博婷; 张可锋

doi:10.13422/j.cnki.syfjx.2017210135

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白马骨水提物对急性肝损伤小鼠氧化应激及炎症反应的影响

药理 | 更新时间：2024-01-04

- 白马骨水提物对急性肝损伤小鼠氧化应激及炎症反应的影响
- Effect of Water Extracts from on Oxidative Stress and Inflammatory Reaction in Mice with Acute Liver Injury
- 中国实验方剂学杂志 2017年23卷第21期页码：135-140
- 作者机构：
- 作者简介：
- 基金信息：
  
  广西重点学科壮药学项目（桂教科研[2013]16号）;广西八桂学者中药创新理论与药效研究项目（J13162）;壮瑶药协同创新中心项目（桂教科研[2013]20号）;广西壮瑶药重点实验室项目（桂科基字[2014]32号）
- DOI：10.13422/j.cnki.syfjx.2017210135
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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高雅, 王刚, 杜沛霖, 等. 白马骨水提物对急性肝损伤小鼠氧化应激及炎症反应的影响[J]. 中国实验方剂学杂志, 2017,23(21):135-140.

GAO Ya, WANG Gang, DU Pei-lin, et al. Effect of Water Extracts from on Oxidative Stress and Inflammatory Reaction in Mice with Acute Liver Injury[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(21): 135-140.
高雅, 王刚, 杜沛霖, 等. 白马骨水提物对急性肝损伤小鼠氧化应激及炎症反应的影响[J]. 中国实验方剂学杂志, 2017,23(21):135-140. DOI： 10.13422/j.cnki.syfjx.2017210135.

GAO Ya, WANG Gang, DU Pei-lin, et al. Effect of Water Extracts from on Oxidative Stress and Inflammatory Reaction in Mice with Acute Liver Injury[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(21): 135-140. DOI： 10.13422/j.cnki.syfjx.2017210135.

摘要

目的：基于氧化应激及炎症反应，研究白马骨水提物对急性肝损伤小鼠的保护作用机制。方法：将60只小鼠随机分为正常组，模型组，阳性组（水飞蓟素0.2 g·kg-1）和白马骨高、中、低剂量（4，2，1 g·kg-1·d-1）组，每组10 只。连续灌胃给药7 d，末次给药2 h 后，除正常组外，其余各组小鼠均腹腔注射0.12% 四氯化碳（CCl4）花生油溶液（10 mL·kg-1），建立小鼠急性肝损伤模型。16 h后，眼球取血，并收取肝脏。生化法检测血清中丙氨酸氨基转移酶（ALT），天门冬氨酸氨基转移酶（AST），总胆红素（TBIL），直接胆红素（DBIL），总超氧化物歧化酶（T-SOD），丙二醛（MDA），谷胱甘肽过氧化物酶（GSH-Px）以及一氧化氮（NO）水平；酶联免疫吸附法（ELISA）检测肝组织中肿瘤坏死因子-α（TNF-α），白细胞介素-1β（IL-1β）和IL-6水平；蛋白质印迹法（Western blot）分析肝组织中核转录因子-κB（NF-κB）蛋白的表达情况；显微观察肝组织病理变化。结果：与正常组比较，模型组血清中ALT，AST，TBIL，DBIL，MDA及NO的水平显著升高，T-SOD和GSH-Px的活性降低（P < 0.01）；肝组织中IL-1β，TNF-α，IL-6含量升高和NF-κB蛋白表达增强（P < 0.01）。与模型组比较，白马骨水提物各剂量组不同程度降低化学性肝损伤小鼠血清中ALT，AST，TBIL，DBIL，MDA及NO的水平（P < 0.05，P < 0.01），并抑制T-SOD和GSH-Px活性的降低；白马骨水提物可抑制肝组织IL-1β，TNF-α，IL-6含量的升高和NF-κB蛋白的表达（P < 0.01，P < 0.05）；病理切片观察也表明白马骨水提物能明显改善受损的肝损伤病理特征。结论：白马骨水提物对CCl4致急性肝损伤小鼠具有明显的保护作用，其作用机制可能与抑制氧化应激，抑制肝脏炎症反应相关。

Abstract

Objective: To study the protective effect of Serissa serissoides water extract on acute liver injury in mice based on oxidative stress and inflammatory response. Method: A total of 60 mice were randomly divided into six groups

namely normal group

model group

silymarin group

and high

middle and low-dose S. serissoides water extract (4

1 g·kg-1·d-1) groups

with 10 in each group. Continuous administration lasted for 7 d. Two hours later after the last administration

the mice in the groups except for the normal group were intraperitoneally injected with 0.12% CCl4 peanut oil solution (10 mL·kg-1) to establish the acute liver injury model. The blood was taken from eye sockets

and the liver was collected. Biochemical method was used to detect serum content of alanine aminotransferase (ALT)

aspartate aminotransferase (AST)

total bilirubin (TBIL)

direct bilirubin (DBIL)

total-superoxide dismutase (T-SOD)

malondialdehyde (MDA)

glutathione peroxidase (GSH-Px) and nitric oxide (NO). Tumor necrosis factor alpha (TNF-alpha)

interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) in liver tissues were detected by enzyme-linked immuno sorbent assay (ELISA)

and the protein expression of NF-kappa B in liver tissues was detected by the western blotting. the pathological change in liver tissues was observed through microscopy. Result: Compared with the normal group

the serum activity or contents of ALT

AST

TBIL

DBIL

MDA and NO significantly increased

whereas T-SOD and GSH-Px activity decreased (P < 0.01)

and the hepatic expressions of IL-1β

TNF-α

IL-6 and NF-κB increased in the model group (P < 0.01). After intervention of the water extract of S. serissoides on acute liver injury

the activities and concentrations of ALT

AST

TBIL

DBIL

MDA and NO in serum were decreased (P < 0.01

P < 0.05)

the T-SOD and GSH-Px activity were increased

and the expressions of IL-1β

TNF-α

IL-6 and NF-κB protein expressions in liver were inhibited (P < 0.05

P < 0.01); The pathological changes also showed that water extract of S. serissoides could significantly improve the pathological characteristics of damaged liver. Conclusion: Water extract of S.serissoides has an obvious protective effect on acute liver injury induced by CCl4 in mice

and its mechanism may be related to inhibition of oxidative stress and inflammatory reaction.

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