五味子-淫羊藿混合提取物对-半乳糖致脑衰老小鼠学习记忆能力的改善作用

刘聪; 李宁; 敬舒; 左红香; 王喜斌; 王春梅; 孙靖辉; 张成义; 陈建光; 李贺

doi:10.13422/j.cnki.syfjx.2017210147

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五味子-淫羊藿混合提取物对-半乳糖致脑衰老小鼠学习记忆能力的改善作用

药理 | 更新时间：2024-01-04

- 五味子-淫羊藿混合提取物对-半乳糖致脑衰老小鼠学习记忆能力的改善作用
- Improvement Effect of Schisandrae Chinensis Fructus-Epimedii Wushanensis Folium Extract on Learning-memorizing Ability in -galactose Induced Brain Aged Mice
- 中国实验方剂学杂志 2017年23卷第21期页码：147-152
- 作者机构：
- 作者简介：
- 基金信息：
  
  吉林省科技厅重点科技攻关计划项目（20140204012YY）;吉林省自然科学基金项目（20150101230JC）;吉林市科技局项目（20166018）
- DOI：10.13422/j.cnki.syfjx.2017210147
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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刘聪, 李宁, 敬舒, 等. 五味子-淫羊藿混合提取物对-半乳糖致脑衰老小鼠学习记忆能力的改善作用[J]. 中国实验方剂学杂志, 2017,23(21):147-152.

LIU Cong, LI Ning, JING Shu, et al. Improvement Effect of Schisandrae Chinensis Fructus-Epimedii Wushanensis Folium Extract on Learning-memorizing Ability in -galactose Induced Brain Aged Mice[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(21): 147-152.
刘聪, 李宁, 敬舒, 等. 五味子-淫羊藿混合提取物对-半乳糖致脑衰老小鼠学习记忆能力的改善作用[J]. 中国实验方剂学杂志, 2017,23(21):147-152. DOI： 10.13422/j.cnki.syfjx.2017210147.

LIU Cong, LI Ning, JING Shu, et al. Improvement Effect of Schisandrae Chinensis Fructus-Epimedii Wushanensis Folium Extract on Learning-memorizing Ability in -galactose Induced Brain Aged Mice[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(21): 147-152. DOI： 10.13422/j.cnki.syfjx.2017210147.

摘要

目的：研究五味子与淫羊藿混合提取物（SEE）对D-半乳糖诱导脑衰老小鼠学习记忆的影响，并探讨其相关机制。方法：将ICR小鼠随机分为5组，即空白组（蒸馏水灌胃，生理盐水皮下注射），衰老模型组（蒸馏水灌胃，D-半乳糖220 mg·kg-1皮下注射），150，300，600 mg·kg-1 SEE组（SEE灌胃，D-半乳糖皮下注射）连续给药7周。通过避暗实验及跳台实验观察SEE对小鼠学习记忆能力的影响。应用水溶性四氮唑-1（water soluble tetrazolium-1，WST-1）法及硫代巴比妥酸法检测小鼠脑组织中超氧化物歧化酶（superoxide dismutase，SOD）活力与丙二醛（malonic dialdehyde，MDA）含量；通过实时荧光定量PCR检测小鼠脑组织p19，p53，p21 mRNA表达情况。通过亚油酸-硫氰酸铁法及Oyaizu法对SEE体外抗氧化能力进行检测。结果：避暗实验及跳台实验结果显示300，600 mg·kg-1 SEE可明显提高D-半乳糖诱导的脑衰老小鼠的学习记忆能力（P < 0.05，P < 0.01）；同时，300，600 mg·kg-1 SEE组小鼠脑组织中SOD的活力明显增加（P < 0.05，P < 0.01），MDA含量明显减少（P < 0.05，P < 0.01），脑组织中p19，p53，p21 mRNA表达量明显降低（P < 0.05，P < 0.01）。亚油酸-硫氰酸铁法实验结果显示，反应体系建立36 h后，SEE对亚油酸的氧化抑制强于维生素E；Oyaizu实验结果显示，SEE对Fe3+的还原能力优于维生素E，吸光度达到0.711。结论：本研究明确了SEE能够改善D-半乳糖致脑衰老小鼠的学习记忆能力，该作用可能与其提高小鼠抗氧化能力以及降低小鼠脑组织中p19，p53，p21基因表达水平有关。

Abstract

Objective: To investigate the effect of Schisandrae Chinensis Fructus-Epimedii Wushanensis Folium extract (SEE) on the learning-memorizing ability in D-galactose induced brain aged mice and explore its relative mechanisms. Method: The ICR mice were divided into five groups randomly

including blank control group (gavage administration of distilled water

subcutaneous injection of normal saline)

aging model group (gavage administration of distilled water

subcutaneous injection of 220 mg·kg-1 D-galactose)

and three SEE groups (150

300

600 mg·kg-1

gavage administration of SEE

orally

subcutaneous injection of D-galactose).All mice were treated for 7 weeks.By using step-through test and step-down test

the effects of SEE on learning-memorizing ability in mice were observed.Water soluble tetrazolium-1 (WST-1) and thiobarbituric acid methods were used to detect the activities of superoxide dismutase (SOD) and malonic dialdehyde (MDA) in the brain tissues of mice. Real-time PCR was used to detect the expressions of p19

p53 and p21 mRNA in the brain

and linoleic acid-ferric thiocyanate method and Oyaizu method were used to test the antioxidation in vitro of SEE. Result: Step-through test and step-down test results showed that 300

600 mg·kg-1 SEE could significantly improve the learning-memorizing ability (P < 0.05

P < 0.01)

increase SOD activities and decrease content of MDA (P < 0.05

P < 0.01)

and decrease the expressions of p19

p53 and p21 mRNA significantly in D-galactose induced brain aged mice (P < 0.05

P < 0.01).Linoleic acid-ferric thiocyanate method results showed that SEE could inhibit oxidation of linoleic acid after establishment of reaction system for 36 hours and its inhibition effect was higher than vitamin E. Oyaizu experiment showed that the reducing capacity of SEE on Fe3+ was stronger than vitamin E

with absorbance of 0.711. Conclusion: The present study demonstrated that SEE could improve the learning-memorizing ability in D-galactose induced brain aged mice

and this effect may be related to its antioxidative activity and decrease of expressions of p19

p53 and p21 mRNA in the brain.

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