Objective: To investigate the mechanism of Aconiti Lateralis Radix Praeparata-Zingiberis Rhizoma in the treatment of heart failure. Method: Protein-protein interaction network related to heart failure was constructed

and targets related to 24 active chemical components from Aconiti Lateralis Radix Praeparata-Zingiberis Rhizoma were predicted. Active compound-predicted target network

active compound-potential target network related to heart failure

and potential target-pathway network were constructed to explore the mechanism of Aconiti Lateralis Radix Praeparata-Zingiberis Rhizoma in the treatment of heart failure. Result: The active compound-potential target network of Aconiti Lateralis Radix Praeparata-Zingiberis Rhizoma related to heart failure contained 23 targets

in which key targets involved Carbonic anhydrase 2 (CA2)

Corticosteroid 11-beta-dehydrogenase isozyme 1 (HSD11B1)

stromelysin-1 (MMP-3)

peptidyl-prolyl cis-trans isomerase FKBP1A (FKBP1A)

cAMP-specific 3

5-cyclic phosphodiesterase 4D (PDE4D)

and heat shock protein HSP 90-alpha (HSP90AA1). In Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis

there were 7 KEGG pathways

mainly involving Renin-angiotensin system. Conclusion: The findings preliminarily verify and predict the basic pharmacological effects and relevant mechanisms of Aconiti Lateralis Radix Praeparata-Zingiberis Rhizoma in the treatment of heart failure

in order to lay a solid foundation for further studies on the mechanism of Aconiti Lateralis Radix Praeparata-Zingiberis Rhizoma in the treatment of heart failure.