不同提取方式对醋乳香中主要乳香酸类成分含量及其抗炎活性的影响

宁张弛; 宋志前; 刘元艳; 王淳; 甘嘉荷; 刘春生; 马新玲; 舒一崧; 刘振丽

doi:10.13422/j.cnki.syfjx.2017220014

您当前的位置：

首页 >

文章列表页 >

不同提取方式对醋乳香中主要乳香酸类成分含量及其抗炎活性的影响

药剂与炮制 | 更新时间：2024-01-04

- 不同提取方式对醋乳香中主要乳香酸类成分含量及其抗炎活性的影响
- Effect of Different Extraction Methods on Content of Main Boswellic Acids in Vinegar-processed Olibanum and Its Anti-inflammatory Activity
- 中国实验方剂学杂志 2017年23卷第22期页码：14-19
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然基金项目面上项目（81470177）;中央级科研院所自主选题（YZ-1656）。
- DOI：10.13422/j.cnki.syfjx.2017220014
  中图分类号： R283.6
- 纸质出版日期：2017
- 稿件说明：
移动端阅览
宁张弛, 宋志前, 刘元艳, 等. 不同提取方式对醋乳香中主要乳香酸类成分含量及其抗炎活性的影响[J]. 中国实验方剂学杂志, 2017,23(22):14-19.

NING Zhang-chi, SONG Zhi-qian, LIU Yuan-yan, et al. Effect of Different Extraction Methods on Content of Main Boswellic Acids in Vinegar-processed Olibanum and Its Anti-inflammatory Activity[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(22): 14-19.
宁张弛, 宋志前, 刘元艳, 等. 不同提取方式对醋乳香中主要乳香酸类成分含量及其抗炎活性的影响[J]. 中国实验方剂学杂志, 2017,23(22):14-19. DOI： 10.13422/j.cnki.syfjx.2017220014.

NING Zhang-chi, SONG Zhi-qian, LIU Yuan-yan, et al. Effect of Different Extraction Methods on Content of Main Boswellic Acids in Vinegar-processed Olibanum and Its Anti-inflammatory Activity[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(22): 14-19. DOI： 10.13422/j.cnki.syfjx.2017220014.

摘要

目的：确定醋乳香挥发油及水提液中是否含有乳香酸类成分，并通过比较乳香酸类成分含量和抗炎活性来确定醋乳香最佳提取方法。方法：采用水提法、乙醇提取法及水蒸气蒸馏-乙醇连续提取法提取，比较6种主要乳香酸类成分α-乳香酸，β-乳香酸，3-乙酰-α-乳香酸，3-乙酰-β-乳香酸，11-羰基-β-乳香酸和11-羰基-3-乙酰-β-乳香酸的含量，以及对脂多糖（LPS）诱导巨噬细胞RAW264.7炎症模型产生白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的含量，探讨醋乳香的最佳提取方法。采用HPLC测定6种乳香酸类成分的含量，流动相0.1%磷酸水溶液-乙腈梯度洗脱，检测波长210 nm和250 nm。结果：在醋乳香挥发油中检测到4种乳香酸类成分，但提取率较低（1.73%~13.02%），水提液中含有上述6种乳香酸类成分。相同提取条件下，乙醇提取法的效率最高，6种成分的提取率81.09%~88.38%。水提法、乙醇提取法及水蒸气蒸馏-乙醇连续提取法细胞液中TNF-α质量浓度分别为（2.248 5±0.332 2），（1.933 2±0.422 9），（2.148 3±0.534 3） μg ·L-1，IL-1β质量浓度分别为（25.53±3.23），（23.22±3.24），（24.32±4.32） ng ·L-1。最佳乙醇提取工艺为加8倍量95%乙醇回流提取2次，每次30 min，各成分的提取率均>90%。结论：醋乳香挥发油中含有少量乳香酸类成分，水提液中也含有乳香酸类成分；采用乙醇提取法时，乳香酸类成分的提取率和抗炎活性最强。

Abstract

Objective: To confirm the existence of boswellic acids in essential oil and water extract of vinegar-processed Olibanum

and to determine its optimum extraction method by comparing the anti-inflammatory activity and the content of main boswellic acids. Method: Water extraction

ethanol extraction and steam distillation-ethanol continuous extraction was adopted

contents of six boswellic acids in water extract

ethanol extract and steam distillation-ethanol continuous extract were determined by HPLC

the anti-inflammatory effect was evaluated by in vitro model of RAW264.7 macrophages induced by lipopolysaccharide(LPS). Result: Four boswellic acids can be detected in essential oil with low extraction rate of 1.73%-13.02% and all of six boswellic acids can be extracted by water.In the same extraction conditions

efficiency of ethanol extraction was optimum with extraction rate of 81.09%-88.38%.Optimum ethanol extraction process was as follows:refluxing extracted twice with 8 times the amount of 95% ethanol for 30 min per time;yield of six boswellic acids were >90%.The content of tumor necrosis factor-α(TNF-α) was (1.933 2±0.422 9) μg · L-1 for ethanol extraction

(2.248 5±0.332 2) μg · L-1 for water extraction and (2.148 3±0.534 3) μg · L-1 for steam distillation-ethanol continuous extraction;their interleukin-1β(IL-1β) contents were (23.22±3.24)

(25.53±3.23)

(24.32±4.32) ng · L-1

respectively. Conclusion: The existence of boswellic acids in essential oil and water extract of vinegar-processed Olibanum is confirmed.Ethanol extraction is superior to other extraction methods with the strongest anti-inflammatory activity.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

早期应用大陷胸汤改善急性胰腺炎重症化趋势的临床疗效

麝香通心滴丸治疗冠状动脉慢血流的疗效及对炎症因子的影响

基于16S rDNA测序探讨滋肾通关方对慢性非细菌性前列腺炎大鼠“肠-前列腺”轴的影响

大黄酒蒸前后对便秘模型小鼠肠道菌群及免疫环境的影响

基于血清代谢组学和流式细胞术探讨百药煎不同炮制品及其复方治疗溃疡性结肠炎作用机制