Objective: To establish a simple and efficient method for the preparation of bakuchiol

and study on its antiosteoporotic activity. Method: The powder of Psoraleae Fructus was extracted with ethanol to get the crude extract of bakuchiol

and bakuchiol was purified with D101 macroporous resin.Moreover

antiosteoporotic activity of bakuchiol on the osteoporosis model was evaluated by zebrafish in vivo. Result: Optimum process for the purification of bakuchiol was as follows

the powder of Psoraleae Fructus was soaked in 85% ethanol overnight and the ratio of ethanol to sample was 1∶ 8

it was refluxed thrice for 2 h each time.The crude extract was concentrated by a vacuum rotary evaporator

and it was dissolved with 0.2% NaOH solution

it was adsorbed by D101 macroporous resin column with a flow rate of 1 BV · h-1

washed by 6 BV of water after 4 h and 2 BV of 60% ethanol

4 BV of 95% ethanol was used to wash the column at last

and eluent was concentrated to get bakuchiol.The yield and purity of bakuchiol prepared by the method were 10% and 80% at least

respectively.In addition

the bioassay indicated that it could prevent bone loss of zebrafish induced by prednisolone

and accelerate growth of zebrafish bone. Conclusion: The method combines the advantages of generality

rapidity and simplicity with high purity.Bakuchiol can be developed into drugs and foods for preventing or treating osteoporosis.