Objective: To study the chemical constituents of the whole plant of Liparis nervosa. Method: The herb L. nervosa was crushed into powders and then extracted with 95% EtOH at 85 ℃. After removal of EtOH under reduced pressure

the solvent was recovered to obtain total extract. The extract was then suspended in distilled water and partitioned successively with petroleum ether (PE)

EtOAc

and n-BuOH to get corresponding fractions. Silica gel column chromatography (CC)

Sephadex LH-20 gel CC and semi-preparation HPLC were used for the separation and purification of PE and EtOAc fractions. The structures of the obtained compounds were identified by physicochemical properties and spectra data. Result: Twelve compounds were isolated from PE and EtOAc extracts of L. nervosa and their structures were identified as p-hydroxybenzoic acid (1)

protocatechuate (2)

3-(4-hydroxyphenyl)-2-propenoic acid (3)

(E)-ferulic acid docosyl ester (4)

(Z)-ferulic acid docosyl ester (5)

stigmast-4-ene-3-one (6)

meranzin hydrate (7)

xanthotoxol (8)

auraptenol (9)

stigmasterol-3-O-β-D-glucoside (10)

vanillic acid (11) and p-hydroxybenzaldehyde (12). Conclusion: Compounds 1-10 were obtained from the genus Liparis for the first time

and compound 11 was obtained from L. nervosa for the first time.