Objective: To analyze and identify the chemical constituents of Xuanmai Ganjie granules by ultra-performance liquid chromatography time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Method: The separation was performed on an XB-Bridge BEH C18 column (2.1 mm×100 mm

2.5 μm) with acetonitrile-0.1% formic acid solution as mobile phase for gradient elution. The flow rate was 0.3 mL · min-1. Electrospray ionization source was used to scan and collect data in positive and negative ion modes. Result: According to the high resolution MS spectra data

fragmentation ion information and retention time

33 chemical constituents of Xuanmai Ganjie granules were identified including 9 iridoid glycosides

20 flavonoids

and 4 saponins by comparison with literature reports and MS cleavage mechanism. Conclusion: The established rapid analysis method based on UPLC-Q-TOF-MS could provide technical support for studying the chemical constituents of Xuanmai Ganjie granules and further studies on its material basis.