Objective: To establish the quality control standard for Huoluo Zhitong gel. Method: Thin layer chromatography(TLC) was used for qualitative identification of Cinnamomi Cortex

Aconiti Kusnezoffii Radix and Corydalis Rhizoma in Huoluo Zhitong gel. Gas Chromatograph-Mass Spectrometer-computer(GC-MS)was used to qualitatively identify the volatile oil in the prescription

and high performance liquid chromatography(HPLC) was used to determine the contents of cinnamaldehyde

mesaconitine

hypaconitine and aconitine. Result: The characteristic TLC spots of the testing samples were the same as the spots of the standard samples without any interference from the negative reference substance. Cinnamic aldehyde was the main component in volatile oil

followed by eugenol. Mesaconitine showed a good linear relationship with peak area within the injection amount of 0.48-2.88 μg(r=1)

hypaconitine within the injection amount of 0.16-0.96 μg(r=1)

aconitine within the injection amount of 0.34-2.04 μg(r=1)

and cinnamaldehyde within the injection amount of 0.268-1.608 μg(r=0.999 1). Conclusion: The method has a good separation and it is simple and accurate

which could be used in the quality control of Huoluo Zhitong gel.