Objective: To explore the effect of Jianpi Yichang powder (JP) on protein and mRNA expression levels of heat shock protein 70 (HSP70) in colon tissues of rats with ulcerative colitis (UC). Method: The 60 healthy SPF male SD rats were randomly divided into normal group and model group. The UC models were established by trinitro-benzene-sulfonic acid(TNBS)/ethanol method. Then the successfully modeled rats were randomly divided into the model group

sulfasalazine group (0.3 g ·kg-1)

and JP high

middle and low dose groups (204

136 and 68 g ·kg-1) with 10 rats in each group. After ig administration for 21 days

the general condition and the colonic mucosa injury of rats were observed. Immunohistochemistry

Western blot and Real-time PCR were used to detect the protein and mRNA expression levels of HSP70 in colon tissues of rats. Result: As compared with the normal group

the colon mucosal injury score was increased significantly (P<0.01)

and the protein and mRNA expression levels of HSP70 were significantly decreased (P<0.01) in model group. As compared with the model group

the colon mucosal injury scores in each drug group were significantly lower (P<0.01)

and the protein and mRNA expression levels of HSP70 were significantly increased (P<0.05

P<0.01). The effect was most obvious in JP high dose group (P<0.05

P<0.01). Conclusion: JP may promote the expression of HSP70 to protect intestinal mucosal immunity in UC rats

so as to play a therapeutic role.