羚贝止咳糖浆止咳、平喘作用及其作用机制

李秀丽; 刘同彦; 李苹; 张翠艳; 陈志国; 徐殿达; 王雅萌; 王玉莹; 杨静娴

doi:10.13422/j.cnki.syfjx.2017220149

您当前的位置：

首页 >

文章列表页 >

羚贝止咳糖浆止咳、平喘作用及其作用机制

药理 | 更新时间：2024-01-04

- 羚贝止咳糖浆止咳、平喘作用及其作用机制
- Antitussive and Antiasthmatic Effect and Its Mechanism of Lingbei Zhike Syrup
- 中国实验方剂学杂志 2017年23卷第22期页码：149-154
- 作者机构：
- 作者简介：
- 基金信息：
  
  吉林省科技发展计划项目（20150311113YY）。
- DOI：10.13422/j.cnki.syfjx.2017220149
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
移动端阅览
李秀丽, 刘同彦, 李苹, 等. 羚贝止咳糖浆止咳、平喘作用及其作用机制[J]. 中国实验方剂学杂志, 2017,23(22):149-154.

LI Xiu-li, LIU Tong-yan, LI Ping, et al. Antitussive and Antiasthmatic Effect and Its Mechanism of Lingbei Zhike Syrup[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(22): 149-154.
李秀丽, 刘同彦, 李苹, 等. 羚贝止咳糖浆止咳、平喘作用及其作用机制[J]. 中国实验方剂学杂志, 2017,23(22):149-154. DOI： 10.13422/j.cnki.syfjx.2017220149.

LI Xiu-li, LIU Tong-yan, LI Ping, et al. Antitussive and Antiasthmatic Effect and Its Mechanism of Lingbei Zhike Syrup[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(22): 149-154. DOI： 10.13422/j.cnki.syfjx.2017220149.

摘要

目的：研究羚贝止咳糖浆止咳平喘效果及通过减轻肺组织病理性凋亡达到止咳平喘作用的实验研究。方法：将48只豚鼠随机分为模型组，阳性药组（川贝雪梨糖浆、氢溴酸右美沙芬组），羚贝止咳糖浆低、中、高剂量（1.27，2.55，5.1 g ·kg-1）组，每组8只，通过豚鼠枸橼酸引咳实验考察羚贝止咳糖浆镇咳作用。将60只豚鼠随机分为模型组，阳性药组（川贝雪梨糖浆、氨茶碱组），羚贝止咳糖浆低、中、高剂量（1.27，2.55，5.1 g ·kg-1）组，每组10只，通过豚鼠氯化乙酰胆碱、磷酸组胺混合引喘实验考察羚贝止咳糖浆平喘作用。取健康小鼠68只，利用烟叶和刨花各50 g早晚各熏蒸1次，1次30 min，连续熏蒸7 d，建立小鼠支气管炎模型，通过苏木素-伊红（hematoxylin and eosin，HE）染色法判定模型建立成功，小鼠支气管炎模型建立成功后通过免疫荧光组织化学法（immunofluorescent histochemistry，IHC）比较空白组、模型组、川贝雪梨糖浆组及羚贝止咳糖浆低、中、高剂量（2.14，4.28，8.56 g ·kg-1）组，肺部组织病理性凋亡相关蛋白B细胞淋巴瘤/白血病-2（B celllymphoma/lewkmia-2，Bcl-2），自杀相关因子（factorassociatedsuicide，Fas）的积分吸光度，研究羚贝止咳糖浆对小鼠支气管炎模型肺部组织病理性凋亡相关蛋白Bcl-2，Fas蛋白表达量的影响。结果：羚贝止咳糖浆显著延长豚鼠咳嗽潜伏期，减少咳嗽次数。延长豚鼠引喘潜伏期。HE染色结果显示成功建立小鼠急性支气管炎模型，免疫荧光化学法结果显示，羚贝止咳糖浆可显著增加炎症肺组织Bcl-2蛋白，减少Fas蛋白的表达。结论：羚贝止咳糖浆通过调控肺部组织凋亡相关蛋白Bcl-2，Fas抑制肺部组织病理性凋亡，帮助恢复组织正常结构减轻肺组织炎症发挥止咳平喘作用。

Abstract

Objective: To study the antitussive and antiasthmatic effect of Lingbei Zhike syrup and conduct the experimental study on reducing lung tissue's pathological apoptosis to reach the antiasthmatic effect. Method: The 48 guinea pigs were randomly divided into model group

positive control group (Chuanbei Xueli syrup

Dextromethorphan Hydrobromide group)

Lingbei Zhike syrup low

medium and high dose groups (1.27

2.55

5.1 g · kg-1)

n=8 in each group. Citric acid-induced cough experiment in guinea pig was used to investigate the antitussive effect of Lingbei Zhike syrup. The 60 guinea pigs were randomly divided into model control group

positive control group (Chuanbei Xueli syrup

aminophylline group)

Lingbei Zhike syrup low

medium and high dose groups (1.27

2.55

5.1 g · kg-1)

n=10 in each group. Acetylcholine-histamine phosphate-induced asthma experiment was used to investigate the antiasthmatic effect of Lingbei Zhike syrup. In 68 healthy mice

50 g tobacco leaves and wood shavings were fumigated once respectively in the morning and evening

30 min/time for continuous 7 days to establish bronchitis models. Hematoxylin and eosin (HE) staining was used to determine whether the models were successfully established. After successful modeling

immunofluorescence histochemistry (IHC) method was used to compare the blank control group

model control group

Chuanbei Xueli syrup group

and Lingbei Zhike syrup low

middle and high dose groups (2.14

4.28

8.56 g · kg-1) in lung tissue pathological apoptosis related protein B cell lymphoma/leukemia-2 (Bcl-2) and integral optical density value of factorassociatedsuicide (Fas)

so as to investigate the effects of Lingbei Zhike syrup on apoptosis related protein Bcl-2 and Fas protein expression levels in lung tissues of bronchitis models. Result: Lingbei Zhike syrup could significantly prolong cough incubation period of guinea pigs and decrease the frequency of cough. HE staining showed that the mouse models of acute bronchitis were successfully established. Immunohistochemistry results showed that Lingbei Zhike syrup could significantly increase the expression level of Bcl-2 protein in lung tissues with inflammation and reduce the expression of Fas protein. Conclusion: Lingbei Zhike syrup could inhibit lung tissue's pathological apoptosis through regulating the expression of apoptosis-related proteins Bcl-2 and Fas

thus helping to restore the normal tissue structure and reduce lung tissue inflammation in playing antiasthmatic effects.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

防己黄芪汤对脑缺血再灌注模型大鼠脑损伤的保护作用

犀角地黄汤干预PKM2介导的一碳代谢途径调控脓毒症炎症反应的机制

线粒体质量控制调控肌少症发病机制及其中医药干预的研究进展

当归芍药散加味调控JNK/p38 MAPK通路改善痤疮炎症和凋亡的机制

子宫内膜异位症程序性细胞死亡和中医药干预作用研究进展