Objective: To establish the HPLC-PDA multiwavelength detection method for the simultaneous determination of citric acid

5-hydroxymethyl furfural (5-HMF) and neochlorogenic acid contents in Method: XBridgeTM C18 (4.6 mm×250 mm

5 μm) column was used with (A) with 0.5% ammonium biphosphate solution (pH 3.0)-acetonitrile (B) as the mobile phase for gradient elution (0-10 min

95%-80%A; 10-15 min

80%-70%A; 15-16 min

70%-95%A; 16-21 min 95%A). The flow rate was 0.8 mL·min-1

and the column temperature was 25℃. The detection wavelength was set at 210

284 and 327 nm. Result: The good linear relationships was shown within 63.30-8 100.00 mg·L-1 (r=0.999 9) for citric acid

0.50-32.00 mg·L-1 (r=0.999 9) for 5-hydroxymethyl furfural

and 0.19-12.00 mg·L-1 (r=0.999 8) for neochlorogenic acid. The recovery of each component was in the range of 97.21%-101.01% and the RSD was less than 2.0%. The three kinds of indicator components in the test solution showed good stability at least within 24 h. The average mass fraction of citric acid

5-HMF and neochlorogenic acid in Mume Fructus Pulp from different origins was 318.71

1.99

2.01 mg·g-1 respectively

and the quality of Mume Fructus Pulp in Zhejiang and Sichuan was the best. Conclusion: This method is accurate and rapid

with low cost

so it can be used for the determination of the three components in Mume Fructus Pulp

providing a reference for quality control in the food and medicine development of ebony.