消脂汤对FFA诱导NASH细胞模型的影响及其相关机制

朱青; 温天燕; 杨道炬; 王晓歌; 袁冬生

doi:10.13422/j.cnki.syfjx.2017230109

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消脂汤对FFA诱导NASH细胞模型的影响及其相关机制

药理 | 更新时间：2024-01-04

- 消脂汤对FFA诱导NASH细胞模型的影响及其相关机制
- Effect and Molecular Mechanisms of Xiaozhi Decoction on NASH Cell Model Induced by FFA in HepG2 Cell
- 中国实验方剂学杂志 2017年23卷第23期页码：109-115
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省省级科技计划项目（2014A020212596）
- DOI：10.13422/j.cnki.syfjx.2017230109
  中图分类号： R285
- 纸质出版日期：2017
- 稿件说明：
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朱青, 温天燕, 杨道炬, 等. 消脂汤对FFA诱导NASH细胞模型的影响及其相关机制[J]. 中国实验方剂学杂志, 2017,23(23):109-115.

ZHU Qing, WEN Tian-yan, YANG Dao-ju, et al. Effect and Molecular Mechanisms of Xiaozhi Decoction on NASH Cell Model Induced by FFA in HepG2 Cell[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(23): 109-115.
朱青, 温天燕, 杨道炬, 等. 消脂汤对FFA诱导NASH细胞模型的影响及其相关机制[J]. 中国实验方剂学杂志, 2017,23(23):109-115. DOI： 10.13422/j.cnki.syfjx.2017230109.

ZHU Qing, WEN Tian-yan, YANG Dao-ju, et al. Effect and Molecular Mechanisms of Xiaozhi Decoction on NASH Cell Model Induced by FFA in HepG2 Cell[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(23): 109-115. DOI： 10.13422/j.cnki.syfjx.2017230109.

摘要

目的：探讨消脂汤对游离脂肪酸（FFA）诱导的非酒精性脂肪性肝炎（NASH）细胞模型的作用及机制。方法：用0.5 μmol·L-1 FFA诱导HepG2细胞建立NASH细胞模型，用消脂汤及非诺贝特干预NASH细胞模型；油红O染色观察细胞内脂滴含量；酶联免疫吸附试验（ELISA）检测细胞上清液中肿瘤坏死因子-α（TNF-α），白细胞介素-6（IL-6）含量；蛋白免疫印迹法（Western blot）检测核转录因子-κB（NF-κB） p65，IκB激酶（IKKβ），IκB磷酸化激酶[Phospho-IKKα/β（Ser176/180）]，胰岛素受体底物1磷酸化蛋白[Phospho-IRS-1（Ser307）]，胰岛素受体底物-1（IRS-1）蛋白表达。结果：FFA刺激24 h后，模型组内脂滴含量，细胞上清液中TNF-α，IL-6含量高于正常组（P<0.01），IKKβ，p-IKKα/β（Ser176/180），p-IRS-1（Ser307），NF-κB（核蛋白）表达明显增加（P<0.01），IRS-1蛋白表达减少（P<0.05），各给药组p-IKKα/β（Ser176/180），p-IRS-1（Ser307），NF-κB p65（核蛋白）表达减少（P<0.01），尤其是消脂汤高浓度组较非诺贝特p-IKKα/β（Ser176/180），p-IRS-1（Ser307）蛋白表达显著减少（P<0.01），各给药组IRS-1蛋白表达明显增加（P<0.05）。结论：消脂汤可改善FFA诱导的NASH细胞模型中脂质沉积及炎症反应，其机制可能与抑制细胞内IKKα/β，IRS-1 Ser307的磷酸化及IKKβ，NF-κB p65（核蛋白）表达有关。

Abstract

Objective: To investigate the effect and mechanism of Xiaozhi decoction on free fatty acids (FFA)-induced non-alcoholic steatohepatitis (NASH) cell model. Method: The NASH model in HepG2 cells was induced by 0.5 μmol·L-1 FFA. The NASH cell models were established by different concentrations of Xiaozhi decoction and fenofibrate. Oil red O staining was used to observe the content of lipid droplets. Cell supernatant (nuclear factor-κ-gene binding) NF-κB p65

IkappaB kinase beta (IKKβ)

Phospho-IκB kinase alpha/beta[Phospho-IKKα/β (Ser176/180)]

Phospho-insulin receptor substrate[Phospho-IRS-1 (Ser307)] and insulin receptor substrate-1 (IRS-1) protein were detected by Western blot. Result: The contents of tumor necrosis factor-alpha (TNF-α) and interleukin-6(IL-6) in the supernatant fluid of the model group were significantly higher than those in control group (P<0.01). Meanwhile

the expression of IRS-1 was reduced (P<0.05)

while the levels of IKKβ

p-IKKα/β (Ser176/180) and IRS-1(Ser307) phosphorylation and nuclear translocation of NF-κB p65 protein were increased in the model group (P<0.01). The expressions of IKKα/β (Ser176/180) and IRS-1 (Ser307) phosphorylation and nuclear translocation of NF-κB p65 protein in each dose group were decreased (P<0.01)

especially in high-dose Xiaozhi decoction. The protein expression of IRS-1 was significantly increased (P<0.05) in control group. Conclusion: Xiaozhi decoction can improve lipid deposition and inflammatory reaction in NASH cell model induced by FFA. The mechanism may be related to the inhibition of intracellular IKKα/β (Ser176/180) and IRS-1 (Ser307) phosphorylation and IKKβ

NF-κB nuclear protein expression.

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