基于成分敲出/敲入模式辨识藜蒿叶抗白色念珠菌的药效物质

庞晨; 贤欢; 李慧; 潘思轶; 徐晓云

doi:10.13422/j.cnki.syfjx.2017240028

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基于成分敲出/敲入模式辨识藜蒿叶抗白色念珠菌的药效物质

药剂与炮制 | 更新时间：2024-01-04

- 基于成分敲出/敲入模式辨识藜蒿叶抗白色念珠菌的药效物质
- Effective Constituents from Leaves Against by Constituent Knock-out/Knock-in Strategy
- 中国实验方剂学杂志 2017年23卷第24期页码：28-35
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家公益性行业（农业）科研专项（201303079）
- DOI：10.13422/j.cnki.syfjx.2017240028
  中图分类号： R285
- 纸质出版日期：2017
- 稿件说明：
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庞晨, 贤欢, 李慧, 等. 基于成分敲出/敲入模式辨识藜蒿叶抗白色念珠菌的药效物质[J]. 中国实验方剂学杂志, 2017,23(24):28-35.

PANG Chen, XIAN Huan, LI Hui, et al. Effective Constituents from Leaves Against by Constituent Knock-out/Knock-in Strategy[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(24): 28-35.
庞晨, 贤欢, 李慧, 等. 基于成分敲出/敲入模式辨识藜蒿叶抗白色念珠菌的药效物质[J]. 中国实验方剂学杂志, 2017,23(24):28-35. DOI： 10.13422/j.cnki.syfjx.2017240028.

PANG Chen, XIAN Huan, LI Hui, et al. Effective Constituents from Leaves Against by Constituent Knock-out/Knock-in Strategy[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(24): 28-35. DOI： 10.13422/j.cnki.syfjx.2017240028.

摘要

目的：基于成分的敲出/敲入模式辨识藜蒿叶水提物中抗白色念珠菌功效的主要活性物质。方法：对藜蒿叶水提物中可溶性成分进行初步检测分析，并以HPD600型大孔树脂对藜蒿叶水提物进行分离，基于成分敲出/敲入模式，用30%乙醇，50%乙醇，70%乙醇水溶液洗脱所得不同组分及其对应阴性样品作为研究对象，采用微量液基稀释法辨识藜蒿叶水提物中对抗菌功效贡献最大的活性组分，并结合HPLC-MS进行初步的物质成分分析。结果：藜蒿叶水提物可溶性成分中糖、蛋白等营养物质质量分数占78%左右，多酚类化合物占16%左右。藜蒿叶水提物经HPD600型大孔树脂，70%乙醇水溶液洗脱后所得组分C是藜蒿叶中主要的抗白色念珠菌（Candida albicans）药效组分，其抗白色念珠菌活性呈剂量依赖效应；质谱分析出组分C主要成分为丹参二萜醌类和萘啶酸类化合物，包括脱氢丹参酮ⅡA，丹参酮ⅡA和丹参酮Ⅰ，以及3种萘啶酸衍生物，以上物质均为首次从藜蒿植株中发现。结论：利用成分敲出/敲入模式辨别分析了藜蒿叶水提物中抗白色念珠菌的主要药效组分，为进一步研究藜蒿叶水提物具有抑菌活性的单体化合物提供了理论依据。

Abstract

Objective: To recognize the main effective antifungal components against Candida albicans in Artemisia selengensis leaves. Method: The soluble components in water extract of A. selengensis leaves were preliminarily detected and analyzed

and then separated by HPD600 macroporous resin. Based on the knock-out/knock-in model

30% ethanol

50% ethanol

and 70% ethanol water solution were used to obtain different components and with their corresponding negative samples as the research object. Then the anti-fungal components in A. selengensis leaves were recognized by microdilution method

and HPLC-MS was combined for preliminary components analysis. Result: The contents of sugar

protein and other nutrients in water soluble components from water extract of A. selengensis accounted for about 78%

and polyphenols accounted for about 16%. The fraction C (70% ethanol elution) from A. selengensis leaves via HPD600 macroporous resin purification was the main anti-fungal component against C. albicans in a dose-effect dependence manner. Mass spectrometry analysis showed that fraction C was mainly of diterpenoid tanshinones and nalidixic acid compounds

including dehydrogenated tanshinone ⅡA

tanshinone ⅡA and tanshinone Ⅰ

as well as 3 kinds of nalidixic acid derivatives. The above substances were discovered from the A. selengensis for the first time. Conclusion: The main effective anti-fungal components against C. albicans in A. selengensis leaves were studied by constituents knock-out/knock-in strategy

providing the theoretical basis for the further study of monomeric compounds with antimicrobial activity from A. selengensis leaves extract.

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