黄柏及黄柏炭饮片的HPLC特征图谱比较

苏慧; 岳琳; 刘颖; 霍雨佳; 扶垭东; 李丽; 王成永

doi:10.13422/j.cnki.syfjx.2017240041

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黄柏及黄柏炭饮片的HPLC特征图谱比较

药剂与炮制 | 更新时间：2024-01-04

- 黄柏及黄柏炭饮片的HPLC特征图谱比较
- Comparison on HPLC Specific Chromatograms Between Phellodendri Chinensis Cortex and Charred Phellodendri Chinensis Cortex Decoction Pieces
- 中国实验方剂学杂志 2017年23卷第24期页码：41-45
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家科技基础性科研专项重点项目（2014FY111100）
- DOI：10.13422/j.cnki.syfjx.2017240041
  中图分类号： O657.72;R284.1
- 纸质出版日期：2017
- 稿件说明：
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苏慧, 岳琳, 刘颖, 等. 黄柏及黄柏炭饮片的HPLC特征图谱比较[J]. 中国实验方剂学杂志, 2017,23(24):41-45.

SU Hui, YUE Lin, LIU Ying, et al. Comparison on HPLC Specific Chromatograms Between Phellodendri Chinensis Cortex and Charred Phellodendri Chinensis Cortex Decoction Pieces[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(24): 41-45.
苏慧, 岳琳, 刘颖, 等. 黄柏及黄柏炭饮片的HPLC特征图谱比较[J]. 中国实验方剂学杂志, 2017,23(24):41-45. DOI： 10.13422/j.cnki.syfjx.2017240041.

SU Hui, YUE Lin, LIU Ying, et al. Comparison on HPLC Specific Chromatograms Between Phellodendri Chinensis Cortex and Charred Phellodendri Chinensis Cortex Decoction Pieces[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(24): 41-45. DOI： 10.13422/j.cnki.syfjx.2017240041.

摘要

目的：建立黄柏及黄柏炭饮片的HPLC特征图谱，分析黄柏饮片炒炭前后特征图谱的变化规律。方法：采用HPLC分析黄柏及黄柏炭饮片的特征图谱，色谱条件为ZORBAX SB-C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈（A）-0.05 mol· L-1磷酸二氢钾溶液（B）（磷酸调pH 3.4）梯度洗脱（0~5 min，5%~10% A；5~20 min，10%~14% A；20~50 min，14%~32% A；50~80 min，32%~70% A），检测波长215 nm，柱温35℃，流速1.0 mL· min-1，进样量10 μL。结果：11批黄柏饮片中均标示出16个特征峰，并归属了其中的9个色谱峰，分别为绿原酸、隐绿原酸、盐酸黄柏碱、木兰花碱、盐酸药根碱、盐酸巴马汀、盐酸小檗碱、黄柏内酯、黄柏酮。通过对色谱峰的分析，黄柏炭仅能检出黄柏中的3个共有峰，且峰面积相差较大；另外标示出了2个特征峰，其中相对比例较高的特征峰经分析验证，确认其为盐酸小檗碱的加热分解产物——小檗红碱。结论：黄柏饮片炒炭前后的特征图谱存在显著差异。该方法准确可靠、重复性好，为黄柏和黄柏炭饮片的质量标准制定提供参考依据。

Abstract

Objective: To establish the HPLC specific chromatograms of Phellodendri Chinensis Cortex decoction pieces and charred Phellodendri Chinensis Cortex decoction pieces

and to study on the change law of HPLC specific chromatograms of Phellodendri Chinensis Cortex before and after being processed. Method: HPLC was employed with the ZORBAX SB-C18 column

acetonitrile-0.05 mol·L-1 potassium dihydrogen phosphate solution (phosphoric acid regulated pH to 3.4) was employed as a mobile phase in gradient elution

the detection wavelength was 215 nm

column temperature was at 35℃ and the flow rate was 1.0 mL·min-1. Result: Eleven batches of Phellodendri Chinensis Cortex decoction pieces were marked with sixteen characteristic peaks

and chlorogenic acid

cryptochlorogenic acid

phellodendrine

magnoflorine

jatrorrhizine hydrochloride

palmatine hydrochloride

berberine hydrochloride

obaculatone and obakunone were assigned.Based on the analysis of characteristic peaks

only three common peaks were detected in charred Phellodendri Chinensis Cortex decoction pieces

and the area of these peaks was quite different;in addition

two feature peaks were identified

with the relative high proportion of the characteristic peak being analyzed and verified to be berberrabine

the product of the heat decomposition of berberine hydrochloride. Conclusion: There are significant differences in the specific chromatograms of Phellodendri Chinensis Cortex decoction pieces before and after being processed.The method is accurate and reproducible

which can provide the basis for the quality standard of Phellodendri Chinensis Cortex decoction pieces and charred Phellodendri Chinensis Cortex decoction pieces.

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