Objective: To determine the content of acteoside in tuberous roots and leaves of different cultivars of Rehmannia glutinosa during their different growth periods at genuine producing areas

and investigate the changes in dynamic accumulation. Method: The acteoside contents from tuberous roots and leaves in 85-5

Beijing-1

Qinhuai

Jingjiu and Baixuan that were cultivated in 2015 and 2016 at Wenxian county were investigated by HPLC method during different harvest periods. Furthermore

acteoside content was detected in tuberous roots and leaves of 85-5 after shading treatments in 2014. Result: There were great differences of acteoside content in tuberous roots and leaves among the different cultivars of R. glutinosa. Acteoside contents were 0.023%-0.620% and 0.018%-0.796% in tuberous roots of Baixuan and Qinhuai respectively

which were higher than that of other cultivars. However

85-5 and Beijing-1 had higher content of acteoside in leaves

ranging from 1.955%-5.968% and 0.681%-5.941%. The contents of acteoside were higher in unfolded leaves and senescing leaves than tender leaves. Shading improved its content in tuberous roots and restrained the accumulation of acteoside in leaves. Conclusion: The results provide the experimental materials and basis for breeding the high-acteoside-content R. glutinosa cultivars

and lay foundations for studying the regulation mechanisms of acteoside biosynthesis in R. glutinosa.