Objective: To establish HPLC fingerprints and spectrum-effect relationships of different batches of Filifolium sibiricum

clarify the contribution of the chemical constituents represented by the common peaks to the antimicrobial efficacy

and reveal the pharmacological basis. Method: Fingerprint was established by HPLC method with acetonitrile-PBS (0.1 mol·L-1 sodium dihydrogen phosphate and 2% acetic acid mixed 1:1) as the mobile phase for gradient elution and the detection wavelength was 360 nm. The inhibitory effects of different samples of F. sibiricum on Staphylococcus aureus

Escherichia coli

methicillin resistant S. aureus

Bacillus paratyphoid and S. albus were studied to reflect their antibacterial activity. Spectrum-effect relationship was revealed by multivariate linear regression and grey relational analysis method. Result: The result of multiple linear regression analysis showed that five antibacterial group experiments (S. aureus

E. coli

methicillin resistant S. aureus

B. paratyphoid and S. albus) were developed respectively and nine peaks per group were correlated with antibacterial activities. The results of gray correlation analysis indicated that isorhamnetin-3-O-rutinoside

isoorientin and isovitexin were correlated with methicillin resistant S. aureus; E. coli and S. aureus were correlated with isorhamnetin-3-O-β-D-glucoside

scopoletin

myricetin

galuteolin

eriocitrin and isoquercitrin; S. albus was correlated with luteolin and quercetin 3-O-rutinoside-7-O-glucoside; and B. paratyphoid was correlated with eriocitrin

mangiferin

myricetin

rutin

scopoletin and isorhamnetin-3-O-β-D-glucoside. The above inhibitory correlation degrees were all more than 0.8. Conclusion: The established HPLC fingerprint method was simple and reproducible.