地黄饮子水煎剂对去势骨质疏松大鼠骨强度和股骨病理形态结构的影响

颜春鲁; 安方玉; 刘永琦; 宋敏; 陈丽; 马正民; 牛彦强

doi:10.13422/j.cnki.syfjx.2017240148

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地黄饮子水煎剂对去势骨质疏松大鼠骨强度和股骨病理形态结构的影响

药理 | 更新时间：2024-01-04

- 地黄饮子水煎剂对去势骨质疏松大鼠骨强度和股骨病理形态结构的影响
- Effect of Dihuang Yinzi Decoction on Bone Strength and Femur Pathological Morphology in Rats by Ovariectomy
- 中国实验方剂学杂志 2017年23卷第24期页码：148-152
- 作者机构：
- 作者简介：
- 基金信息：
  
  甘肃省高等学校科研项目（2015A-097）;甘肃中医药大学教学研究与教学改革项目（ZH-201606）;甘肃省高校重大疾病分子医学与中医药防治研究重大实验室开放基金项目（FZYK15-16-7）
- DOI：10.13422/j.cnki.syfjx.2017240148
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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颜春鲁, 安方玉, 刘永琦, 等. 地黄饮子水煎剂对去势骨质疏松大鼠骨强度和股骨病理形态结构的影响[J]. 中国实验方剂学杂志, 2017,23(24):148-152.

YAN Chun-lu, AN Fang-yu, LIU Yong-qi, et al. Effect of Dihuang Yinzi Decoction on Bone Strength and Femur Pathological Morphology in Rats by Ovariectomy[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(24): 148-152.
颜春鲁, 安方玉, 刘永琦, 等. 地黄饮子水煎剂对去势骨质疏松大鼠骨强度和股骨病理形态结构的影响[J]. 中国实验方剂学杂志, 2017,23(24):148-152. DOI： 10.13422/j.cnki.syfjx.2017240148.

YAN Chun-lu, AN Fang-yu, LIU Yong-qi, et al. Effect of Dihuang Yinzi Decoction on Bone Strength and Femur Pathological Morphology in Rats by Ovariectomy[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(24): 148-152. DOI： 10.13422/j.cnki.syfjx.2017240148.

摘要

目的：研究地黄饮子水煎剂对去势大鼠骨强度和骨病理形态结构的影响，进一步探讨其作用机制。方法：采用去卵巢法制备大鼠骨质疏松症模型，将大鼠随机分为假手术组，模型组，地黄饮子水煎剂低、中、高剂量组，西药组（戊酸雌二醇片），每组10只，分别予相应药物灌胃4周，股动脉采血处死大鼠。观察大鼠的一般状况，称量体重，比色分析法检测血清钙（calcium，Ca），磷（phosphorus，P）含量，酶联免疫吸附法（ELISA）检测血清骨钙素（osteocalcin，BGP）含量和抗酒石酸酸性磷酸酶（tartrate resistant acid phosphatase，trACP）含量，双能X射线吸收测量仪平台上测定右股骨部位骨密度（bone mineral density，BMD），用AG-IX生物力学万能实验机检测股骨最大载荷，定位-伸长位移及苏木素-伊红（HE）染色法观察股骨的病理形态学变化。结果：与假手术组比较，模型组大鼠BMD，Ca，P，BGP，最大载荷，定伸长-位移均显著降低（P<0.05），而模型组大鼠体重和trACP的含量及地黄饮子水煎剂各干预组大鼠体重则显著增加（P<0.05），同时模型组骨小梁结构不完整、丢失、断裂严重。与模型组相比，地黄饮子水煎剂各干预组大鼠BMD，BGP，Ca，最大载荷，定伸长-位移均显著升高（P<0.05），其高、中剂量组大鼠P含量显著升高，而其高剂量组大鼠体重和trACP的含量则显著降低（P<0.05），并且地黄饮子水煎剂高剂量组骨小梁排列规则，数目增多、增宽。结论：地黄饮子能够提高绝经后骨质疏松大鼠的骨密度，促进骨形成、抑制骨吸收，对绝经后骨代谢异常具有积极的防治作用。

Abstract

Objective: To study the effects of Dihuang Yinzi decoction on bone strength and femur pathological morphology of ovariectomized rats

to further explore its mechanism. Method: Cutting off ovaris on both sides made rat model of osteoporosis

all animals were randomly separated into sham operation group

model control group

Dihuang Yinzi decoction high

medium

low dose group

western medicine control group(estradiol valerate tablets)

with 10 rats in each group. The rats were gavaged with corresponding drugs for 4 weeks. And weight method was monitor the weight body of each rats. The general statu of the rats was evalued

the contents of Ca and P was used to check by colorimetric analysis

the contents of BGP and trACP were used to check by ELISA

bone mineral density(BMD) was used to obtain data by X ray dual energy absorptiometer

maximum load and fixed elongation displacement was used to obtain data by AG-IX biomechanical universal testing machine

the morphological changes of the femur was used to observe by HE staining. Result: Compared with those of sham operation group

the BMD

BGP

maximum load and fixed elongation displacement were all decreased obviously (P<0.05)

but the weight body and trACP activity were both markedly increased (P<0.05)

as well as

trabecular bone structure was incomplete

missing and broken seriously. Compared with those of model control group

the BMD

BGP

maximum load and fixed elongation displacement were all raised sharply in dihuangyinzi decoction treatment group

the P content was increased sharply in dihuangyinzi decoction high

middle dose group(P<0.05)

but the weight body and trACP activity were both significantly slowed in Dihuang Yinzi decoction high dose group (P<0.05)

meantime

bone trabecula was arranged regularly

bone trabecula was increased in number and widthin Dihuang Yinzi decoction high dose group. Conclusion: Dihuang Yinzi could improve bone density and inhibit bone formation

reduce bone absorption in rats with postmenopausal osteoporosis

and lead to a positive effect on the treatment of postmenopausal abnormal bone metabolism.

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