Objective:To optimize the DNA extraction method and molecular identification of Angelicae Sinensis Radix preparations by analyzing trnL-F sequences and using the Angelicae Sinensis Radix specific marker. Method:DNA was extracted by the traditional cetyltrimethylammonium bromide (CTAB) method

sodium dodecylsulphate (SDS) method

paramagnetic particle method and modified CTAB method from 10 kinds of Angelicae Sinensis Radix preparations. The integrity

purity

and concentration of the total genomic DNA yielded by the four methods were detected by ultraviolet spectrophotometry and agarose gel electrophoresis. The plastid trnL-F of 10 samples were amplified

sequenced and analyzed

and the phylogenetic trees were constructed. The DNA samples were also applied for molecular identification with Angelicae Sinensis Radix specific marker. Result:The modified CTAB method was found to yield relatively pure and high quality DNA from the 10 kinds of Angelicae Sinensis Radix preparations. The similarity of 10 samples and Angelicae Sinensis Radix ranged from 99.88% to 100%. Phylogenetic tree based on trnL-F sequences showed that 10 samples and Angelicae Sinensis Radix were clustered as one category. The clear and bright amplified band was obtained for all 10 samples in the verification by Angelicae Sinensis Radix specific molecular marker. Conclusion:The genomic DNA in Angelicae Sinensis Radix preparations could be successfully extracted by the modified CTAB method. The A. sinensis were successfully identified by the trnL-F sequences and the Angelicae Sinensis Radix specific molecular marker

with great importance in the molecular identification and quality control in Angelicae Sinensis Radix preparations.