Objective:To quantitatively analyze quercitrin-3-O-β-D-glucose-(1→6)-β-D-glucoside (M1)

quercitrin-3-O-[2"-O-acetyl-β-D-glucose-(1→6)-β-D-glucoside] (M2)

and isoquercitrin (M3) in 11 batches of flowers of Meconopsis integrifolia by ultra performance liquid chromatography (UPLC). Method:The ultrasonic extraction method was used with processing time of 45 min

and the extraction solvent was 90% methanol aqueous solution. ACQUITY UPLC HSS T3 chromatographic column was used at the column temperature of 35℃

flow rate of 300 μL·min-1 and the detection wavelength of 255 nm

with acetonitrile-0.1% glacial acetic acid solution (16:84) as the mobile phase for isocratic elution for 10 min. Result:The contents of 3 flavonoids in 11 batches of flowers of M. integrifolia in different regions and at different acquisition time were different. Generally

the contents of quercitrin were higher than the content of isoquercitrin; the total content of flavonoid glycosides in Mengbi mountain

Maerkang county was higher than that in the other regions; and the content of flavonoid glycosides from the samples collected in May and June were higher than those collected in July. Conclusion:The contents of main components in 11 batches of flowers of M. integrifolia were relevant to their origins and collection time. The method in this study could reflect the exact contents of compositions

providing reference for quality evaluation and quality standard improvement of M. integrifolia.