Objective:To discuss the effect of Shenling Baizhusan on Ghrelin and Obestatin protein expression of in the rat models with chronic obstructive pulmonary disease (COPD)

and investigate its mechanism and scientific connotation in treating COPD based on the Ghrelin/Obestatin signal regulating pathway from the molecular level. Method:The 60 SPF Wistar rats were randomly divided into blank control group

lung and spleen deficiency model group and Shenling Baizhusan groups (28 days group

35 days group and 42 days group according to different drug intervention time)

12 in each group. Lung and spleen deficiency COPD rat models were established by using the disease and syndrome combination method. In blank control group

the rats inhaled air

intratracheally injected with the same amount of normal saline

and orally took the same amount of normal saline. The rats in model group and Shenling Baizhusan groups were treated with smoking+intratracheal lipopolysaccharide+oral administration of ice and cold senna leaf infusion to build pulmonary and spleen deficiency type COPD rat model. Shenling Baizhusan was given by intragastric administration

and the rats were sacrificed at 28

35

42 days after drug intervention. Immunohistochemistry and Western blot were used to detect the expression changes of Ghrelin and Obestatin in hypothalamus and gastric tissues of rats. Result:As compared with normal blank group

the Ghrelin and Obestatin protein expression and integral absorbance in stomach and hypothalamus were significantly decreased in model group (P<0.01). As compared with model group

the Ghrelin and Obestatin protein expression and integral absorbance in stomach and hypothalamus were increased in different degrees in Shenling Baizhusan groups (P<0.05). Conclusion:The Shenling Baizhusan can regulate the energy metabolism of the lung and spleen deficiency type COPD rats and improve the malnutrition condition. Its mechanism may be associated with the regulation of Ghrelin and Obestatin dynamic balance to maintain body energy homeostasis.