Objective:To observe the effect of Tangshenqing No.1 high

medium and low doses (traditional Chinese medicine) on the expression of group CC cytokines in renal tissues of rats with diabetic nephropathy. Method:Diabetic nephropathy rat model was established

and then the rats were divided into normal control group

model control group

Captopril group

Tangshenqing No.1 high

medium and low dose treatment groups. The rats in normal control group and model control group received intragastric administration of 10 mL·kg-1 deionized water daily; captopril 2.33 mg·kg-1 was given daily in Captopril group; Tangshenqing No.1 high

medium and low dose groups respectively received 187

93.5

46.75 mg·kg-1 Tangshenqing No.1.Samples were taken after 12 weeks of successive administration. The mRNA transcription of monocyte chemoattractant protein-1(MCP-1) and regulated upon activation normal T cell expressed and secreted (RANTES) in renal tissues were detected by Real-time polymerase chain reaction (Real-time PCR). The expression level of MCP-1 protein was detected by Western blot

while the expression level of RANTES protein was determined by enzyme linked immunosorbent assay (ELISA). Result:Tangshenqing No.1 could down-regulate the mRNA transcription and protein expression levels of MCP-1 and RANTES in renal tissues (P<0.05

P<0.01) in a dose-dependent manner. The expression levels of MCP-1 and RANTES in renal tissues were as follows:Tangshenqing No.1 high dose group < middle dose group < low dose group. The curative effect was most obvious in Tangshenqing No.1 high dose group among the Chinese medicine treatment groups. Conclusion:Tangshenqing No.1 can inhibit the occurence and development of diabetic nephropathy by inhibiting the expression of MCP-1

RANTES mRNA and protein in renal tissues.