Objective:To explore the protective effect and mechanism of Micrococtis Folinm on liver of Mouse with non-alcoholic fatty liver disease (NAFLD). Method:BALB/c mouse were randomly divided into normal group

model group

simvastatin group (2.5 mg·kg-1) and high and low-dose Micrococtis Folinm groups (3.5

7.0 g·kg-1). The NAFLD model was prepared through intragastric administration with high-sugar and high-fat diets for 12 weeks

once a day. Serum triglyceride (TG)

cholesterol (TC)

high-density lipoprotein cholesterol (HDL-C)

low density lipoprotein cholesterol (LDL-C)

alanine amino transaminase (ALT) and aspartate aminotransferase (AST) were detected

and liver index were calculated (liver wet weight/body weight×100%) at the 28th day. Activity of superoxide dismutase (SOD) and malondialdehyde (MDA) in liver homogenate were determined by xanthine oxidase and thiobarbital acid method

respectively. Adiponectin receptor 2 (Adipo R2) in liver tissues was detected by western blotting. The morphological changes in liver tissues were observed under light microscope. Result:Compared with the normal group

the model group showed higher levels of serum TC

TG

LDL-C

AST and ALT (P<0.01)

and a lower level of serum HDL-C (P<0.01)

higher liver index (P<0.01)

lower SOD activity (P<0.01) and higher MDA (P<0.01) in liver homogenate

decreased Adipo R2 protein expression in liver tissues (P<0.05)

and severer hepatic steatosis (P<0.01). Compared with the model group

the Micrococtis Folinm group showed lower levels of serum TC

TG

LDL-C

AST and ALT (P<0.01)

lower liver index (P<0.01)

lower MDA (P<0.01) and increased SOD activity (P<0.01) in liver homogenate

increased Adipo R2 protein expression in liver tissues (P<0.05)

and significantly relieved liver steatosis (P<0.01). Conclusion:Micrococtis Folinm has lipid-lowering and hepatoprotective effects on NAFLD mice

and its mechanism may be related to the inhibition of oxidative stress and the increase of Adipo R2 expression.